Ray development defects in lin-32 and Wnt/β-catenin asymmtery mutantsa
| Genotypeb | Rays per side | RnAs (trp-4::mCh) per side | RnBs (pkd-2::GFP) per side | n (sides) |
|---|---|---|---|---|
| Wild-type | 9.0 ± 0.3 | 6.2 ± 1.5 | 7.9 ± 0.5 | 64 |
| lin-32(tm2044Δ) | 2.3 ± 1.0*** | 2.0 ± 1.0*** | 3.3 ± 1.0*** | 88 |
| lin-17(n671) | 0.0*** | n.d. | n.d. | 18 |
| lin-17(n698) | 3.7 ± 2.5*** | 7.2 ± 3.4** | 5.6 ± 3.5*** | 110 |
| lin-17(bx109) | 6.8 ± 2.5*** | 6.7 ± 2.3 | 7.2 ± 3.0 | 78 |
| lin-44(n1792) | 4.5 ± 2.0 | 4.0 ± 2.4 | 7.2 ± 1.6 | 36 |
| lin-44(n1792); egl-20(n585) | 3.2 ± 2.0̂ | 5.5 ± 1.6̂̂ | 7.3 ± 1.2 | 38 |
| lin-44(n1792); cwn-1; cwn-2 | 3.6 ± 2.1 | 5.2 ± 1.1̂ | 7.4 ± 1.6 | 32 |
| Control RNAi | 8.9 ± 0.4 | n.d. | n.d. | 58 |
| lit-1(RNAi) | 6.6 ± 1.7††† | n.d. | n.d. | 93 |
| sys-1(RNAi) | 8.1 ± 1.1††† | n.d. | n.d. | 94 |
| pop-1(RNAi)c | 3.9 ± 2.3††† | n.d. | n.d. | 100 |
n.d., Not determined. **p < 0.01, ***p < 0.001 compared with wild-type controls. *p < 0.05, **p < 0.01 compared with lin-44(n1792).
†††p < 0.001 compared with control RNAi.
aAll values are reported as mean ± SD.
bAll strains contain him-5(e1490) (see Materials and Methods). Except for the RNAi experiments, strains listed here also contained the transgene fsIs18.
cMany pop-1(RNAi) animals had large “fused” rays that could arise from either the fusion of two separate rays (as a result of a defect in ray identity specification) or the development of multiple Rnst ray structural cells from a single ray sublineage.