Primary antibodies, dilutions, and sources used in experiments (see Materials and Methods), a sample reference in which the specificity of the antibody has been demonstrated, and the method used to validate the specificity of the antibody
| Molecule | Species | Dilution | Source | Specificity references | Validation method |
|---|---|---|---|---|---|
| PV | Mouse IgG | 1:10,000 1:3000 (EM) | Swant, PV-28 | Celio et al., 1988 | Immunoblot of brain and muscle lysates |
| Rabbit IgG (used for PVCre mice) | 1:1000 | Calbiochem, PC255L | Mithani et al., 1987 | Western blot of rat brain and muscle lysates | |
| SYP | Mouse IgM | 1:5000 | Millipore, mab sp15 | Honer et al., 1993 | Immunoblot of human brain lysates |
| Cav3.1 | Rabbit IgG | 1:100 | Alomone, ACC-021 | Ernst et al., 2009 | Western blot of brain lysates from transgenic mice overexpressing receptor; validated against Cav3.1 KO mouse (T. Snutch, personal communication) |
| TAU1 | Mouse IgG | 1:5000 | Millipore, mab3420 | Binder et al., 1985 | Immunoblot of rat brain lysates |
| ChAT | Goat IgG | 1:1000 | Millipore Bioscience Research Reagents, AB144P | Raised against human placental enzyme; company tested | Western blot of mouse brain lysates |