Table 1.

Extracellular and intracellular solutions used for the electrophysiological recordings

Composition (mm) of extracellular solutions, pH adjusted to 7.4 with NaOH
NaClNaGlucNaSCNKClKGlucCaCl2MgCl2HEPESTEA-ClSucroseGlucose
ExtCl-131102.720221.210201514
ExtCl-10139.1221.2103.61514
ExtSCN9132.5221.2101514
Composition (mm) of intracellular solutions, pH adjusted to 7.2 with KOH
KSCNKClKGlucEGTACaCl2MgCl2HEPESTEA-ClMgATPSucroseVj (mV)
IntCl-131107.111111020214−2.2
IntCl-10121.11111106214−11.4
IntCl-15111.111111011235−12.8
IntCl-4121.1111110252−14.6
IntSCN132.5111110214
  • The junction potential (Vj) was calculated for the different intracellular solutions with reference to ExtCl-131 using Clampex 8.1 and a temperature of 21°C. Gluc, Gluconate; Ext, extracellular; Int, intracellular.