Composition (mm) of extracellular solutions, pH adjusted to 7.4 with NaOH | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
NaCl | NaGluc | NaSCN | KCl | KGluc | CaCl2 | MgCl2 | HEPES | TEA-Cl | Sucrose | Glucose | |
ExtCl-131 | 102.7 | 20 | 2 | 2 | 1.2 | 10 | 20 | 15 | 14 | ||
ExtCl-10 | 139.1 | 2 | 2 | 1.2 | 10 | 3.6 | 15 | 14 | |||
ExtSCN | 9 | 132.5 | 2 | 2 | 1.2 | 10 | 15 | 14 |
Composition (mm) of intracellular solutions, pH adjusted to 7.2 with KOH | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
KSCN | KCl | KGluc | EGTA | CaCl2 | MgCl2 | HEPES | TEA-Cl | MgATP | Sucrose | Vj (mV) | |
IntCl-131 | 107.1 | 11 | 1 | 1 | 10 | 20 | 2 | 14 | −2.2 | ||
IntCl-10 | 121.1 | 11 | 1 | 1 | 10 | 6 | 2 | 14 | −11.4 | ||
IntCl-15 | 111.1 | 11 | 1 | 1 | 10 | 11 | 2 | 35 | −12.8 | ||
IntCl-4 | 121.1 | 11 | 1 | 1 | 10 | 2 | 52 | −14.6 | |||
IntSCN | 132.5 | 11 | 1 | 1 | 10 | 2 | 14 |
The junction potential (Vj) was calculated for the different intracellular solutions with reference to ExtCl-131 using Clampex 8.1 and a temperature of 21°C. Gluc, Gluconate; Ext, extracellular; Int, intracellular.