Kinetic constants | IPSCs mean amplitude (pA) | Quantum content (m) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
τ rise 20–80% (ms) | τ decay 10–90% (ms) | n | sIPSCs | n | eIPSCs | n | (1) Mean eIPSC/mean sIPSC | (2) ln(N/N0) | (3) 1/CV2 | |
1 | 11.8 ± 4.0 | 56.7 ± 21.3 | 238 | 58 ± 18 | 285 | 34 ± 16 | 48 | 0.05 | 0.07 | 0.06 |
2 | 14.4 ± 5.6 | 67.6 ± 22.1 | 35 | 59 ± 21 | 40 | 99 ± 48 | 276 | 1.15 | 1.17 | 1.25 |
3 | 14.1 ± 4.9 | 58.0 ± 21.8 | 173 | 52 ± 25 | 201 | 62 ± 38 | 188 | 0.48 | 0.51 | 0.41 |
4 | 9.2 ± 3.6 | 44.0 ± 14.3 | 88 | 47 ± 21 | 119 | 35 ± 21 | 103 | 0.14 | 0.21 | 0.16 |
5 | 10.3 ± 3.8 | 41.2 ± 12.2 | 48 | 48 ± 23 | 54 | 51 ± 30 | 116 | 0.11 | 0.14 | 0.11 |
6 | 12.3 ± 4.0 | 49.2 ± 14.8 | 57 | 35 ± 17 | 68 | 28 ± 14 | 40 | 0.03 | 0.05 | 0.04 |
7 | 11.2 ± 3.5 | 32.3 ± 8.7 | 31 | 28 ± 15 | 133 | 39 ± 25 | 285 | 0.31 | 0.23 | 0.17 |
8 | 17.2 ± 6.6 | 60.4 ± 22.6 | 70 | 42 ± 20 | 117 | 39 ± 23 | 68 | 0.21 | 0.26 | 0.20 |
Mean ± SD | 12.6 ± 2.6 | 51.2 ± 11.6 | 46.1 ± 10.8 | 48.4 ± 23.1 | 0.31 ± 0.37 | 0.35 ± 0.37 | 0.31 ± 0.40 |
eIPSCs were evoked by stimulation at 1 Hz, while sIPSCs were recorded in the absence of stimulation. In the first two rows, 20–80% rise time and 10–90% decay time for the sIPSCs are shown, while the fourth and sixth rows show the mean amplitude of eIPSCs and sIPSCs, respectively, for eight OHCs. In each cell, the quantum content (m) was estimated in three different ways: (1) dividing the mean eIPSC amplitude (including failures in the mean) by the mean sIPSC amplitude (direct method); (2) by the rate of failures, with m = ln(N/N0), where N is the number of electrical stimuli and N0 is the number of failures (method of failures); or (3) by the coefficient of variation (CV) method, with m = 1/CV2. Values are the mean ± SD.