Analyses | MPTP time course | ||||||
---|---|---|---|---|---|---|---|
MPTP | +1 | +14 | +21 | +28 | +35 d | +42 d | |
DAT immunoreactivity (FI, % of control) | 100 ± 15 | 22 ± 8* | 25 ± 10* | 38 ± 9* | 51 ± 9* | 65 ± 10* | 75 ± 12 |
[3H]DA uptake (% of control) | 100 ± 18 | 31 ± 10* | 29 ± 8* | 35 ± 10* | 45 ± 10* | 58 ± 12* | 68 ± 12 |
Male C57BL/6 mice received n = 4 intraperitoneal injections of vehicle (saline, 10 ml/kg) or MPTP-HCl (20 mg kg−1 free base; Sigma-Aldrich) dissolved in saline, 2 h apart in 1 d. For each set of analyses, five mice were used for the indicated time points. The brains were processed as indicated for DAT immunohistochemistry. Fluorescence intensity values (means ± SEM) are expressed as percentage of saline. Synaptosomial high-affinity [3H]DA uptake in striatum (total high-affinity and mazindol noninhibitable) was determined as described, and the values are represented by the changes in DA uptake (expressed as percentage of control). Differences were analyzed by ANOVA, followed by the Newman–Keuls test, and were considered significant when p < 0.05.
↵*p < 0.05 versus MPTP.