Rescue phenotypes of cortical synapses with complexin-1/2 double knockdown expressing the indicated complexin forms
| Complexin used for rescue | Vesicle priming | Vesicle clamping | Fast Ca2+ triggering |
|---|---|---|---|
| No rescue | ↓ | ↓ | ↓ |
| Complexin-1 | ↑ | ↑ | ↑ |
| Complexin-3 | ↑ | ↓ | ↑ |
| Complexin-11–86 | ↓ | ↓ | ↑ |
| Complexin-1/3 hybrid | n.d. | ↓ | ↑ |
| Complexin-3/1 hybrid | n.d. | ↑ | ↑ |
| Complexin-1I101W | n.d. | ↑ | ↑ |
| Complexin-1L117W | n.d. | ↑ | ↑ |
| Complexin-1L117K | n.d. | ↓ | ↑ |
| Complexin-1V120P | n.d. | ↓ | ↑ |
Summary of the complexin KD and rescue experiment results. Complexin-1/2 KD results in reduced vesicle priming and clamping and decreased Ca2+ triggering of fast release. The efficacy of various complexin proteins to rescue the three features of the KD phenotype is indicated (↑, rescue; ↓, no rescue; n.d., not determined).