Cell group | Location | n | No. of cells | ΔF/F0 |
---|---|---|---|---|
NT control | Somata | 18 (6) | 18 (6) | 0.299 ± 0.015 (0.741 ± 0.021) |
Dendritic spines | 34 (11) | 14 (4) | 0.357 ± 0.022 (0.577 ± 0.046) | |
Dendritic shafts | 28 (9) | 14 (5) | 0.368 ± 0.026 (0.603 ± 0.046) | |
MFBs | 64 | 12 | 1.302 ± 0.050 | |
shKIF21A | Somata | 19 (5) | 19 (5) | 0.300 ± 0.018 (0.724 ± 0.043) |
Dendritic spines | 45 (11) | 13 (5) | 0.364 ± 0.029 (0.672 ± 0.047) | |
Dendritic shafts | 35 (11) | 13 (5) | 0.306 ± 0.027 (0.673 ± 0.062) | |
MFBs | 46 | 15 | 2.161 ± 0.079** | |
shNCKX2 | Somata | 7 | 7 | 0.268 ± 0.022 |
Dendritic spines | 15 | 4 | 0.329 ± 0.033 | |
Dendritic shafts | 10 | 4 | 0.358 ± 0.037 | |
MFBs | 26 | 5 | 2.171 ± 0.148** |
[Ca2+]i increments presented as ΔF/F0 of OGB-5N. Ca2+ transients were induced by HFS at 33 or 100 Hz (numbers in parentheses). ΔF/F0 values of a Ca2+ transient were read at the end of HFS. NT, Nontargeting. The statistical values are presented as mean ± SEM.
↵**Statistical significance compared with control value (p < 0.01).