Opioid receptor-mediated [35S]GTPγS binding by TAN67, SNC80, and DAMGO in brain regions of P154 rats consuming either intermittent 20% ethanol or water only for long periods from age P56
| Brain region | Mean (±SEM) EC50 values (μm) for opioid-mediated [35S]GTPγS stimulation | |||||
|---|---|---|---|---|---|---|
| TAN67 | SNC80 | DAMGO | ||||
| 20% ethanol | Water only | 20% ethanol | Water only | 20% ethanol | Water only | |
| Dorsal striatum | 0.03 ± 0.01 | >10 | 18 ± 2.6 | >10 | 0.84 ± 0.02*** | 0.37 ± 0.02 |
| Nucleus accumbens | >10 | >10 | >10 | >10 | 0.32 ± 0.02 | 0.32 ± 0.03 |
| Spinal cord | 3.8 ± 0.4* | 5.0 ± 0.4 | >10 | >10 | 0.56 ± 0.04*** | 0.30 ± 0.02 |
| Cerebral cortex | 3.7 ± 0.6 | 3.5 ± 0.8 | 1.3 ± 0.7 | 2.3 ± 0.8 | 3.0 ± 0.4*** | 0.50 ± 0.08 |
| Brainstem | 5.5 ± 0.3 | 5.9 ± 0.2 | >10 | >10 | 0.07 ± 0.004*** | 0.05 ± 0.002 |
| Midbraina | >10 | >10 | >10 | >10 | 1.4 ± 0.5 | 1.8 ± 0.4 |
| Ventral tegmental area | >10 | >10 | >10 | >10 | 0.28 ± 0.03 | 0.28 ± 0.02 |
| Amygdala | >10 | >10 | >10 | >10 | >10 | 1.4 ± 0.3 |
| Cerebellum | >10 | >10 | >10 | >10 | 0.32 ± 0.08 | 0.18 ± 0.09 |
| Hippocampus | >10 | >10 | >10 | >10 | >10 | >10 |