Isolation of EAAC1 protein from rat kidney
| Detergent | Rat age | Antibody ID | Amount of pure EAAC1 protein obtained (μg) | WGA | DEAE | Sample ID |
|---|---|---|---|---|---|---|
| Cholate | 220–240 g | Ab#237 | 88 | No | Yes | 1374 |
| SDS | 9 weeks | Ab#555 | 12 | Yes | No | 1357 |
| SDS | 9 weeks | Ab#555 | 20 | Yes | No | 1358 |
Rat kidneys were solubilized in cholate or SDS and EAAC1 isolated using immobilized anti-C491 antibodies (either Ab#237 or Ab#555). The bound EAAC1 protein was eluted at low pH, neutralized, and further purified by chromatography on either a wheat germ agglutinin lectin (WGA) column or a DEAE column as indicated. The age is given in weight (grams) or in weeks.