Comparison of sIPSC parameters in WT and α3KO PCs under various conditions
| Genotype | IPSC parameters | ||||
|---|---|---|---|---|---|
| Frequency (Hz) | Amplitude (pA) | Rise time (ms) | t1 (ms) | t2 (ms) | |
| α3KO | |||||
| LA PC (7) | 9.9 ± 1.4* | 32.3 ± 3.4 | 0.99 ± 0.18 | 5.08 ± 0.94 | 10.37 ± 1.13 |
| BLA PC (10) | 18.4 ± 2.5 | 37.3 ± 5.8 | 0.94 ± 0.06 | 4.19 ± 0.59 | 9.21 ± 0.59 |
| 0.5 μm TP003 (5) | 15.5 ± 3.1 | 50.6 ± 8.4 | 0.92 ± 0.05 | 5.12 ± 1.01 | 10.9 ± 2.1 |
| 1 μm diazepam (5) | 16.0 ± 1.8 | 71.0 ± 12.3* | 0.81 ± 0.07 | 7.1 ± 1.3 | 25.4 ± 2.7*** |
| WT | |||||
| LA PC (8) | 9.3 ± 1.7* | 37.2 ± 5.6 | 0.94 ± 0.04 | 5.47 ± 0.49 | 9.08 ± 1.14 |
| BLA PC (13) | 15.0 ± 1.6 | 41.5 ± 3.9 | 0.89 ± 0.05 | 5.02 ± 0.46 | 10.52 ± 0.77 |
| 0.5 μm TP003 (5) | 11.3 ± 2.1 | 43.4 ± 3.5 | 0.88 ± 0.04 | 5.40 ± 1.2 | 12.56 ± 2.2 |
| 1 μm diazepam (5) | 16.6 ± 1.6 | 62.4 ± 1.1** | 0.88 ± 0.07 | 6.8 ± 1.2 | 33.85 ± 8.3*** |
↵Values (mean ± SEM) significantly different from the control (solvent treated BLA principal cells of the respective genotype) are indicated with *p < 0.05, **p < 0.01, and ***p < 0.001 (Student's unpaired t test). Numbers of recorded neurons are given in parentheses. Bold indicates control values.