CNTF activates astrocytes and has limited effects on other neuroinflammation markers
| Gene symbol (common name) | Sequence # | mRNA level | |
|---|---|---|---|
| Lenti-LacZ | Lenti-CNTF | ||
| Astrocytes | |||
| Gfap | NM_017009 | 100 ± 20.95 | 1272.89 ± 144.83** |
| Vim (vimentin) | NM_031140 | 100 ± 23.15 | 830.81 ± 121.58** |
| Microglia | |||
| Itgam (CD11b) | NM_012711 | 100 ± 12.67 | 165.78 ± 34.27 |
| Aif1 (IBA1) | NM_017196 | 100 ± 13.65 | 138.95 ± 12.83 |
| Cytokines and chemokines | |||
| IL-1a | NM_017019 | 100 ± 7.71 | 125.22 ± 26.98 |
| IL-6 | NM_012589 | 100 ± 10.68 | 97.39 ± 14.57 |
| IL-10 | NM_012854 | 100 ± 19.31 | 96.26 ± 21.57 |
| LIF | NM_022196 | 100 ± 15.70 | 82.28 ± 17.17 |
| OSM (oncostatin M) | NM_001006961 | 100 ± 23.39 | 128.47 ± 23.40 |
| Tnfa (TNFα) | NM_012675 | 100 ± 13.04 | 200.56 ± 34.05* |
| Ccl3 (MIP1-α) | NM_013025 | 100 ± 9.53 | 57.53 ± 19.34 |
| Ccl5 (RANTES) | NM_031116 | 100 ± 29.48 | 82.54 ± 34.32 |
| Cxcl1 (GRO/KC) | NM_030845 | 100 ± 17.57 | 61.72 ± 5.24 |
| C3 | NM_016994 | 100 ± 19.54 | 127.33 ± 32.83 |
| Oxidative stress | |||
| Ncf1 (p47phox) | NM_053734 | 100 ± 13.95 | 109.77 ± 17.44 |
| Ncf2 (p67phox) | XM_344156 | 100 ± 22.58 | 95.16 ± 4.84* |
| Nox4 | NM_053524 | 100 ± 23.74 | 95.65 ± 30.43 |
| Noxa1 | XM_231042 | 100 ± 29.41 | 117.65 ± 11.76** |
| Noxo1 | XM_220221 | 100 ± 21.74 | 65.22 ± 21.76 |
| Nos2 (inducible nitric oxide synthase) | NM_012611 | 100 ± 52.05 | 76.64 ± 36.82 |
| Ptgs1 (Cyclooxygenase 1) | NM_017043 | 100 ± 8.62 | 115.52 ± 8.62 |
| Ptgs2 (Cyclooxygenase 2) | NM_017232 | 100 ± 15.46 | 76.81 ± 6.76** |
Analysis by RT-qPCR array of the expression of 22 genes related to neuroinflammation in rats injected with lenti-LacZ or lenti-CNTF. mRNA levels of all genes were normalized to actin mRNA levels and expressed relatively to the LacZ group (set at 100%). n = 5;
↵*p < 0.05, **p < 0.005, paired t test.