Table 1.

qPCR primers

ForwardReverse
18S ribosomal subunitCGGCTACCACATCCAAGGAAGCTGGAATTACCGCGGCT
MRF (total)aGCATGGGCACCGCCCCTAAGGGGGCGAGTCTGGCAGTGTG
MRF (non-recombined)bAAGGAGCTGCCTATGCTCACCTGCCTCTAGCTTCACACCATGCA
MRF (recombined)cATCCATCAAATGGCAGCCGCACAGGGCCTCTAGAGCTCCTT
MAGAACCAGTATGGCCAGAGAGCGTTCCGGGTTGGATTTTACC
PLP1GCCCCTACCAGACATCTAGCAGTCAGCCGCAAAACAGACT
MBPCCCGTGGAGCCGTGATCTCTTCAAACGAAAAGGGA
MOGATGAAGGAGGCTACACCTGCCAAGTGCGATGAGAGTCAGC
Sox10TGGACCGCACACCTTGGGACAACGCCCACCTCCTCCGACCT
CNPGTTCTGAGACCCTCCGAAAACCTTGGGTTCATCTCCAGAA
PDGFRαGGAAGGACTGGAAGCTTGGGGCGAGATGAGGCCCGGCCCTGTGA
CD68GGACTACATGGCGGTGGAGATGAATTCTGCGCCATGAA
β-3-tubulinTCTCGGCCTCGGTGAACTCAAGGCCTTCCTGCACTGGTA

  • ↵aRecognized transcripts arising from both non-recombined and recombined alleles.

  • ↵bSpecific to non-recombined allele.

  • ↵cSpecific to post-recombination/nonfunctional allele.