Table 1.

Oligonucleotides used for site-directed mutagenesis

Construct	Oligonucleotide	Sequence (5′ to 3′)
TAS2R10-S85A	Forward	`CTAATTGAATATATTGCTTACTTTTGGGTAATTG`
	Reverse	`CAATTACCCAAAAGTAAGCAATATATTCAATTAG`
TAS2R10-S85T	Forward	`GGTAACCTAATTGAATATATTACTTACTTTTGGGTAATTGG`
	Reverse	`CCAATTACCCAAAAGTAAGTAATATATTCAATTAGGTTACC`
TAS2R10-S85V	Forward	`GTAACCTAATTGAATATATTGTTTACTTTTGGGTAATTGG`
	Reverse	`CCAATTACCCAAAAGTAAACAATATATTCAATTAGGTTAC`
TAS2R10-W88A	Forward	`CTAATTGAATATATTAGTTACTTTGCGGTAATTGGTAATCAATCAAG`
	Reverse	`CTTGATTGATTACCAATTACCGCAAAGTAACTAATATATTCAATTAG`
TAS2R10-W88F	Forward	`GAATATATTAGTTACTTTTTCGTAATTGGTAATCAATCAAG`
	Reverse	`CTTGATTGATTACCAATTACGAAAAAGTAACTAATATATTC`
TAS2R10-W88H	Forward	`CCTAATTGAATATATTAGTTACTTTCATGTAATTGGTAATCAATCAAG`
	Reverse	CTTGATTGATTACCAATTACATGAAAGTAACTAAT ATATTCAATTAGG
TAS2R10-V89A	Forward	`GAATATATTAGTTACTTTTGGGCAATTGGTAATCAATCAAGTATGTGG`
	Reverse	`CCACATACTTGATTGATTACCAATTGCCCAAAAGTAACTAATATATTC`
TAS2R10-N92A	Forward	`GTTACTTTTGGGTAATTGGTGCTCAATCAAGTATGTGGTTTG`
	Reverse	`CAAACCACATACTTGATTGAGCACCAATTACCCAAAAGTAAC`
TAS2R10-N92D	Forward	`CTTTTGGGTAATTGGTGATCAATCAAGTATGTGGTTTG`
	Reverse	`CAAACCACATACTTGATTGATCACCAATTACCCAAAAG`
TAS2R10-Q93A	Forward	`GTTACTTTTGGGTAATTGGTAATGCATCAAGTATGTGGTTTGCCAC`
	Reverse	`GTGGCAAACCACATACTTGATGCATTACCAATTACCCAAAAGTAAC`
TAS2R10-Q175A	Forward	`GTATAAAAGTGAATACTTTATTAAAGCGATTTTGCTAAATCTGGGAGTC`
	Reverse	`GACTCCCAGATTTAGCAAAATCGCTTTAATAAAGTATTCACTTTTATAC`
TAS2R10-Q175L	Forward	`GTATAAAAGTGAATACTTTATTAAATTGATTTTGCTAAATCTGGGAGTC`
	Reverse	`GACTCCCAGATTTAGCAAAATCAATTTAATAAAGTATTCACTTTTATAC`
TAS2R10-Q175N	Forward	`GTATAAAAGTGAATACTTTATTAAAAATATTTTGCTAAATCTGGGAGTC`
	Reverse	`GACTCCCAGATTTAGCAAAATATTTTTAATAAAGTATTCACTTTTATAC`
TAS2R10-Q175T	Forward	`GTATAAAAGTGAATACTTTATTAAAACGATTTTGCTAAATCTGGGAGTC`
	Reverse	`GACTCCCAGATTTAGCAAAATCGTTTTAATAAAGTATTCACTTTTATAC`
TAS2R10-Q175Y	Forward	`GTGAATACTTTATTAAATACATTTTGCTAAATCTGGGAGTC`
	Reverse	`GACTCCCAGATTTAGCAAAATGTATTTAATAAAGTATTCAC`
TAS2R10-L178A	Forward	`CTTTATTAAACAGATTTTGGCAAATCTGGGAGTCATTTTC`
	Reverse	`GAAAATGACTCCCAGATTTGCCAAAATCTGTTTAATAAAG`
TAS2R10-L178F	Forward	`CTTTATTAAACAGATTTTGTTTAATCTGGGAGTCATTTTCTTC`
	Reverse	`GAAGAAAATGACTCCCAGATTAAACAAAATCTGTTTAATAAAG`
TAS2R10-L178T	Forward	`CTTTATTAAACAGATTTTGACAAATCTGGGAGTCATTTTCTTC`
	Reverse	`GAAGAAAATGACTCCCAGATTTGTCAAAATCTGTTTAATAAAG`
TAS2R10-Y239A	Forward	`CATCATCCTCTTTATCTTGGCTTTTATAGGCATGGCCATAG`
	Reverse	`CTATGGCCATGCCTATAAAAGCCAAGATAAAGAGGATGATG`
TAS2R10-M263A	Forward	`CTTATGTTTGGAGCGACAACCACAG`
	Reverse	`CTGTGGTTGTCGCTCCAAACATAAG`
TAS2R10-M263E	Forward	`CTTATGTTTGGAGAGACAACCACAGCC`
	Reverse	`GGCTGTGGTTGTCTCTCCAAACATAAG`
TAS2R10-T266A	Forward	`GTTTGGAATGACAACCGCAGCCATCTATCCCTG`
	Reverse	`CAGGGATAGATGGCTGCGGTTGTCATTCCAAAC`
TAS2R10-M263E-T266A	Forward	`GTTTGGAGAGACAACCGCAGCCATCTATC`
	Reverse	`GATAGATGGCTGCGGTTGTCTCTCCAAAC`
Vector primer	CMV forward	`CGCAAATGGGCGGTAGGCGTG`
	pCR3.1-BGH reverse	`TAGAAGGCACAGTCGAGG`

The constructs generated by point mutagenesis are specified in the left column. Next, the nucleotide sequences for the forward and reverse primers are given in 5′ to 3′ direction.