Table 1.

Primary afferent glutamatergic innervation of S2 VF neurons with crossed rostral projections

Lamina	VF cells	VF + Aff + VGluT1	Lamina	VF cells	VF + Aff + VGluT2
IV, V	65	11	IV, V	62	13
VII, X	247	51	VII, X	237	48
VIII, IX	63	5	VIII, IX	27	5
Total number	375	67	Total number	326	66

The laminar distribution of S2 VF neurons contacted by sacral primary afferents with VGluT1 (VF + Aff + VGluT1) or VGluT2 (VF + Aff + VGluT2) immunoreactivity. Data are based on retrograde labeling of cut VF axon bundles at the lumbosacral junction, anterograde labeling of the contralateral sacrocaudal afferents entering the S2/S3 dorsal root, and immunostaining for VGluT1 (2 experiments) or VGluT2 (4 experiments). Colocalization was determined only for VF neurons with clear putative SCA contacts using high-power confocal imaging (see Fig. 4), and thus may underestimate the extent of innervation by VGluT IR primary afferents.