Table 1.

Sequences of primers used for real-time RT-PCR assays

Gene	Forward Primer (5′–3′)	Reverse Primer (5′–3′)
ARTN	`GGCCAACCCTAGCTGTTCT`	`TGGGTCCAGGGAAGCTT`
ASIC2a	`ATGGACCTCAAGGAGAGCCCCAG`	`AAGTCTTGATGCCCACACTCCTGC`
ASIC2b	`CGCACAACTTCTCCTCAGTGTTTAC`	`TTGGATGAAAGGTGGCTCAGAC`
GAPDH	`ATGTGTCCGTCGTGGATCTGA`	`ATGCCTGCTTCACCACCTTCTT`
GDNF	`AGCTGCCAGCCCAGAGAATT`	`GCACCCCCGATTTTTGC`
GFRα1	`GTGTGCAGATGCTGTGGACTAG`	`TTCAGTGCTTCACACGCACTTG`
GFRα2	`TGACGGAGGGTGAGGAGTTCT`	`GAGAGGCGGGAGGTCACAG`
GFRα3	`CTTGGTGACTACGAGTTGGATGTC`	`AGATTCATTTTCCAGGGTTTGC`
Nav 1.8	`GCCACCCAGTTCATTGCCTTTTC`	`TCCCCAGATTCTCCCAAGACATTC`
NGF	`ACACTCTGATCACTGCGTTTTTG`	`CCTTCTGGGACATTGCTATCTGT`
NRTN	`TGAGGACGAGGTGTCCTTCCT`	`AGCTCTTGCAGCGTGTGGTA`
P2X₃	`TGGAGAATGGCAGCGAGTA`	`ACCAGCACATCAAAGCGGA`
p75	`GGGTGATGGCAACCTCTACAGT`	`GTGTCACCATTGAGCAGCTTCT`
Piezo 2	`GGCACTAGCATGTGCGTTC`	`TGTCCTTGCATCGTTGCTTTG`
Ret	`TGAGTGCACCAAGCTTCAGTAC`	`GCGAAGCCTGGGTCTGTCT`
Runx1	`TTTCAAGGTACTCCTGCCTGA`	`CAGTGAGAAGGACCAGAGACT`
TrkA	`AGAGTGGCCTCCGCTTTGT`	`CGCATTGGAGGACAGATTCA`
TRPA1	`GCAGGTGGAACTTCATACCAACT`	`CACTTTGCGTAAGTACCAGAGTGG`
TRPM8	`CGTGGGAGGGTGTCATGAAG`	`GTTGTCGTTGGCTTTCGTGTT`
TRPV1	`TTCCTGCAGAAGAGCAAGAAGC`	`CCCATTGTGCAGATTGAGCAT`