Table 2.

Number of synaptic puncta per neuron in the four different co-culture combinations

14 DIV21 DIV
BassoonPSD95ColocalizedBassoonPSD95Colocalized
Awt/wt|Nwt/wt362 ± 28517 ± 43275 ± 251634 ± 791918 ± 991501 ± 73
Ako/ko|Nwt/wt714 ± 49***832 ± 78***569 ± 44***1225 ± 83**1419 ± 94**1121 ± 76**
Awt/wt|Nko/ko511 ± 24619 ± 32378 ± 191222 ± 83**1357 ± 102***1093 ± 75***
Ako/ko|Nko/ko637 ± 52***787 ± 58*510 ± 42*853 ± 51***1105 ± 66***803 ± 51***

  • The expression of Bassoon (presynaptic) and PSD95 (postsynaptic) puncta in neuronal cultures was analyzed using the puncta analyzer plug-in for ImageJ. The values indicate the average number of puncta per neuron that were recorded for the presynaptic (Bassoon), the postsynaptic (PSD95), and the colocalized markers (overlap of Bassoon and PSD95) in a representative experiment. Values are given as the average number of puncta ± SEM. For each condition, 40 neurons were counted, which is n = 160 for each time point and thus n = 320 for 14 DIV and 21 DIV. Differences compared with the Awt/wt|Nwt/wt control situation for a given time point were assessed by ANOVA and significance levels are indicated by asterisks.

  • ↵*p ≤ 0.05,

  • ↵**p < 0.01,

  • ↵***p < 0.001. Overall, 320 individual neurons yielding 960 puncta number counts were evaluated for this table. For comparison of independent experiments, the percent increase or decrease increments were calculated and are presented as a bar graph in Figure 4B,D.