Rescue of lethality in VAChTdel/del mice
| Genotype | Number of mice born |
|---|---|
| VAChTwt/wt | 2 |
| VAChTwt/del | 7 |
| ChAT–ChR2–EYFP VAChTwt/del | 10 |
| ChAT–ChR2–EYFP VAChTdel/del | 4 |
| ChAT–ChR2–EYFP VAChTwt/wt | 6 |
| Total | 29 |
ChAT–ChR2–EYFP mice were crossed to VAChTdel/wt mice and offspring were then intercrossed. Genomic qPCR using primers that amplify the VAChTdel allele was used to identify live VAChTdel/del mice containing the ChaT BAC.