Fear conditioning quantification, Torino protocol
| Less-excit (α6Cacna1a) | Repeated-measures ANOVA WT vs KO | ||
| Day 1 training | 0.11 | ||
| WT, n = 10 | KO, n = 9 | t test | |
| Day 2 baseline (%) | 17.6 ± 5.2 | 19.0 ± 5.0 | 0.85 |
| Day 2 test (%) | 41.6 ± 2.9 | 40.0 ± 2.0 | 0.67 |
| Day 2 baseline vs test | 0.00 | 0.00 | |
| No-inhib (L7-γ2) | Repeated-measures ANOVA WT vs KO | ||
| Day 1 training | 0.85 | ||
| WT, n = 7 | KO, n = 8 | t test | |
| Day 2 baseline (%) | 11.1 ± 4.9 | 10.8 ± 4.7 | 0.97 |
| Day 2 test (%) | 33.7 ± 3.7 | 37.4 ± 6.2 | 0.62 |
| Day 2 baseline vs test | 0.00 | 0.00 | |
| No-LTP (L7-PP2B) | Repeated-measures ANOVA WT vs KO | ||
| Day 1 training | 0.25 | ||
| WT, n = 12 | KO, n = 12 | MW/t test | |
| Day 2 baseline (%) | 22.8 ± 6.4 | 29.1 ± 9.4 | 0.91 |
| Day 2 test (%) | 46.7 ± 3.5 | 47.8 ± 4.7 | 0.86 |
| Day 2 baseline vs test | 0.00 | 0.06 | |
| No-LTD (L7-PKCi) | Repeated-measures ANOVA WT vs TG | ||
| Day 1 training | 0.46 | ||
| WT, n = 14 | TG, n = 12 | MW/t test | |
| Day 2 baseline (%) | 20.5 ± 6.0 | 18.7 ± 6.5 | 0.76 |
| Day 2 test (%) | 49.4 ± 3.8 | 43.3 ± 3.8 | 0.24 |
| Day 2 baseline vs test | 0.00 | 0.00 | |
Mean values ± SEM of the percentage of time spent freezing during cued testing (day 1 baseline and day 2 test; Fig. 6A; see Materials and Methods for protocol details) for the four mouse lines. Statistical differences between genotypes (KO/TG vs WT) for the acquisition curve (day 1, training) were calculated with an ANOVA for repeated measures test. Statistical differences for baseline and test between genotypes (KO/TG vs WT) and within genotypes (successfulness of the test, i.e.. most time spent freezing during test compared with baseline periods) were calculated with a Student's t test for normally-distributed data (regular font) or with a Mann–Whitney test for non-normally distributed data (MW; italic font).