Biochemical analysis
| Mice | Alanine aminotransferase (U/L) | Bilirubin (μmol/L) |
|---|---|---|
| Sham | 21 ± 1.04 | 2.2 ± 0.2 |
| BDL | 531.2 ± 82.2 | 198.2 ± 22 |
| Control IgG-treated BDL | 546.5 ± 60.7 | 184 ± 28.6 |
| Anti-Gr-1-treated BDL | 706 ± 174.5 | 169.0 ± 30.6 |
| Control IgG-treated BDL | 592.29 ± 54.4 | 283.14 ± 38.6 |
| Anti-P-selectin-treated BDL | 622.86 ± 74.7 | 209.71 ± 16.1 |
| PSGL-1 KO BDL | 835 ± 164.2 | 243.3 ± 71.0 |
| WT BDL | 648.3 ± 41.6 | 183 ± 29.7 |
| Anti-TNFα-treated BDL | 592.6 ± 76.4 | 206 ± 25.2 |
| NRS-treated BDL | 576.3 ± 83.6 | 233.5 ± 26.8 |
| TNFR1 KO BDL | 343.0 ± 80.1 | 238.2 ± 32.3 |
| WT BDL | 449.8 ± 93.5 | 167 ± 39.8 |
| TNFR2 KO BDL | 575.7 ± 59.5 | 230 ± 18.9 |
| WT BDL | 571 ± 14.1 | 168 ± 58.3 |
| iNOS KO BDL | 475 ± 64.2 | 327.2 ± 21.6 |
| WT BDL | 447 ± 64.9 | 297.8 ± 25.5 |
| Minocycline-treated BDL | 456.7 ± 158.03 | 127.7 ± 25.1 |
| Control BDL | 518 ± 75.6 | 125.2 ± 18.9 |
| DNBS-treated mice | 23.3 ± 4.1 | <2 |
| Control mice | 21.3 ± 1.3 | <2 |
Alanine aminotransferase and bilirubin levels in sham, BDL, and mice administered different treatments as described. Results expressed as mean ± SEM of data from 5–6 mice per group. BDL, Bile duct ligation; WT, wild-type; NRS, normal rabbit serum; iNOS, inducible nitric oxide synthase; PSGL-1, P-selectin glycoprotein ligand-1; DNBS, dinitrobenzene sulfonic acid.
p < 0.05 versus sham controls. Each treatment group was compared to its own control group. ALT and Bilirubin values for all BDL mice are significantly different from Sham mice.