Projection neurons subpopulations showing retrograde transgene expression with rAAV constructsa
| Projection target | Source | Distance (mm) | Expression locus (-Cre mouse) | Vector |
|---|---|---|---|---|
| CPu | SNC | 3.5 | TH | 2/1.FLEX.GCaMP3 |
| AcbSh | VTA | 4.4 | TH | 2/1.FLEX.GCaMP3 |
| PrL/IL | VTA | 5.0 | TH | 2/1.FLEX.GCaMP3; 2/1.FLEX.tdTomato |
| BLA | DR | 5.4 | SERT | 2/9.DIO.hChR2-EYFP |
| aPC | OB | 2.7 | PCdh21 | See Table 1 |
| pPC | OB | 5.3 | PCdh21 | See Table 1 |
| PLCo | OB | 6.8 | PCdh21 | See Table 1 |
| MeA | AOB | 4.6 | PCdh21 | See Table 1 |
| OB | AON | 2.2 | CCK | 2/1.FLEX.GCaMP3 |
| OB | HDB | 4.3 | GAD65 | 2/1.FLEX.GCaMP5G |
| OB | HDB | 4.3 | ChAT | 2/5.DIO.hChR2-EYFP |
| cS1BF (contra) | S1BF | 6.0 (lateral) | WT, Emx1 (cortical pyramidal neurons) | 2/1.FLEX.GCaMP3; 2/1.FLEX.tdTomato; 2/1.mCherry |
| SC | S1BF | 1.9 | WT, Emx1 (cortical pyramidal neurons) | 2/1.FLEX.GCaMP3; 2/1.mCherry |
| Thalamus | S1BF | 2.5 (diagonal) | WT (cortical pyramidal neurons) | 2/1.mCherry |
↵a Table summarizes virus injection sites and Cre expression loci resulting in retrograde transgene expression after infection by rAAV constructs. Viral constructs were injected into the projection targets of cells located in the source area. Reported distance between the injection site and source area is the straight-line distance in the anterior–posterior direction unless otherwise noted. Expression locus represents mouse line expressing Cre from the designated locus. For WT mice, the targeted neuron types are listed. AOB, Accessory olfactory bulb; BLA, basolateral amygdaloid nucleus, anterior part; CPu, caudate putamen (striatum), DR, dorsal raphe nucleus; MeA, medial amygdaloid nucleus, anterior part; PLCo, posterolateral cortical amygdaloid nucleus; pPC, posterior piriform cortex; S1BF, primary somatosensory cortex, barrel field; SC, superior colliculus; SNC, substantia nigra, pars compacta.