The KCC2–S940D mutant and PD150606 calpain inhibitor prevented the 4-AP-induced increased mobility of synaptic and extrasynaptic KCC2–Flag transporters
| Median D (10−2 μm2s−1) | ||||||
|---|---|---|---|---|---|---|
| Extra | ES | IS | ||||
| Ctrl | 4AP | Ctrl | 4AP | Ctrl | 4AP | |
| S940D | 4.1 (176) | 4.1 (190) | 2.9 (68) | 2.3 (81) | 2.8 (98) | 2.3 (78) |
| PD150606 | 4.5 (139) | 4.8 (118) | 4.1 (72) | 3.4 (44) | 3.6 (85) | 2.7 (53) |
Numbers in parentheses indicate the numbers of QDs analyzed. The number of cells and cultures analyzed are as in Table 4. In all cases, there was no statistical significance between control and 4-AP conditions. Ctrl, Control; Extra, QDs at extrasynaptic sites; ES, QDs at excitatory synapses; IS, QDs at inhibitory synapses.