Table 2.

List of differentially regulated classes of genes in microglia derived from Cd11bCre;EP2lox/lox versus Cd11bCre;EP2+/+ brains

Gene symbolGene nameFold changeGO process/function/molecular component
Immune responseIL1Binterleukin 1, beta−2.04184Cytokine activity
IL1R2interleukin 1 receptor, type II−2.31737Cytokine receptor
Il1f9interleukin family member, 9−2.04786Cytokine activity
CXCR2chemokine (C-X-C motif) receptor 2−1.86059Chemotaxis
CXCR4chemokine (C-X-C motif) receptor 4−1.86059Chemotaxis
THBS1thrombospondin 1−1.95183Cell adhesion
SSP1Osteopontin−1.54035Cell adhesion
ITGALintegrin, alpha L−1.58753Cell adhesion
TROAPtrophinin associated protein−1.75552Cell adhesion
Clec4nC-type lectin domain family 4, member N−1.61132Carbohydrate binding
Clec4dC-type lectin domain family 4, member d−2.1454Carbohydrate binding
KLRD1killer cell lectin-like receptor subfamily D, mbr 1−1.65784Carbohydrate binding
PTGS2prostaglandin-endoperoxide synthase 2 (COX-2)−2.17048Cnflammatory response
TREM1triggering receptor expressed on myeloid cells 1−1.69313Innate immune response
MMP9matrix metallopeptidase 9−1.61825Extracellular matrix organization
PILRBpaired immunoglobin-like type 2 receptor beta−1.81032Myeloid dendritic activation
FPR1formyl peptide receptor 1−1.7669TLR1 signaling pathway
ECM1extracellular matrix protein 1−1.70548Regulation of type 2 immune response
HDChistidine decarboxylase−1.67452Histamine biosynthetic process
Stfa2stefin A2−1.96096Regulator of phospholipase A2 activity
Cfpcomplement factor properdin−1.65784Extracellular matrix
Cell cycleANAPC1anaphase promoting complex subunit 1−2.1456Anaphase promoting complex
ANAPC2anaphase promoting complex subunit 2−2.13443Anaphase promoting complex
CCNA2cyclin A2−1.67413Regulation of cell cycle
CCNB2cyclin B2−1.70104Regulation of cell cycle
CDKN3cyclin-dependent kinase inhibitor 3−1.8019Cell cycle
CENPEcentromere protein E, 312kDa−1.81685M phase of mitotic cycle
CENPFcentromere protein F, 350/400kDa (mitosin)−1.68826M phase of mitotic cycle
NUSAP1nucleolar and spindle associated protein 1−1.82572Mitotic cytokinesis
CEP55centrosomal protein 55kDa−2.07399Mitosis
MKI67antigen identified by monoclonal antibody Ki-67−1.79318Cell proliferation
ASPMasp (abnormal spindle) homolog−1.98094Maintenance of centrosome location
BUB1Bbudding uninhibited by benzimidazoles 1 hom ß−1.8033Metaphase/anaphase transition mitotic cell cycle
SPAG5sperm associated antigen 5−1.72396Mitotic sister chromatic segregation
PRC1protein regulator of cytokinesis 1−1.89456Maintenance of transcriptionally repressed state
Cks2CDC28 protein kinase regulatory subunit 2−1.75907Cell cycle
Nuf2NUF2, NDC80 kinetochore complex component−1.74007Mitosis
STILSCL/TAL1 interrupting locus−1.76871Notch receptor processing, ligand independent
Cytoskeletal functionCKAP2Lcytoskeleton associated protein 2-like−1.71323Component of centrosome
KIF11kinesin family member 11−1.76381Kinesin motor activity
KIF15kinesin family member 15−1.70974Kinesin motor activity
KIF18Bkinesin family member 18B−1.92932Kinesin motor activity
KIF20Akinesin family member 20A−1.92174Kinesin motor activity
KIF22kinesin family member 22−1.82174Kinesin motor activity
KIF2Ckinesin family member 2C−1.88518Kinesin motor activity
TPX2TPX2, microtubule-associated, homolog−1.77056Regulation of mitotic spindle organization
Signal transductionTtkTtk protein kinase−1.68733ser/thr kinase activity
Ect2ect2 oncogene−1.77594Cytokinesis
Arfgef1ADP-ribosylation factor guanine nt-exchange factor−2.14379Vesicle-mediated transport
AURKAaurora kinase A−1.873Mitotic cell cycle
MASTLmicrotubule associated serine/threonine kinase-like−1.94399G2/M transition of mitotic cell cycle
MELKmaternal embryonic leucine zipper kinase−1.74326Protein phosphorylation
NEK2NIMA-related kinase 2−1.91723Mitotic sister chromatid segregation
PIM1pim-1 oncogene−1.92845Cell proliferation
PLK1polo-like kinase 1−1.75168G2/M transition of mitotic cell cycle
RACGAP1Rac GTPase activating protein 1−1.73564Cytokinesis, initiation of separation
PbkPDZ binding kinase−1.68555Protein phosphorylation
ProteolysisHUWE1HECT, UBA and WWE domain 1, E3 Ub protein ligase−2.38982Protein ubiquitination
Itchitchy, E3 ubiquitin protein ligase−1.92047Protein ubiquitination
MetabolicSult2a7sulfotransferase family 2A, dehydroepiandrosterone−1.72082Sulfotransferase activity
ESDesterase D−1.77798Formaldehyde catabolic process
Exocytosis RNA processingExoc7exocyst complex component 72.01344Exocytosis
Tbl3transducin (beta)-like 31.98707rRNA processing
Rrn3RRN3 RNA polymerase I transcription factor homolog1.93842Positive regulation of transcription
Pabpn1poly(A) binding protein, nuclear 12.47361mRNA polyadenylation
TranscriptionDEPDC1DEP domain containing 1−1.79393Transcriptional repressor/signal transduction
NFKBIDNFkB inhibitor, delta−1.65827Negative regulation of NFkB activity
Chromatin assemblyH2AFXH2A histone family, member X−1.75055Nucleosome assembly
Hist1h2aghistone cluster 1, H2ag−1.78355Nucleosome assembly
Hist1h2abhistone cluster 1, H2ab−2.1247Nucleosome assembly
  • Listed are genes with a known Gene Ontology (GO pathway) process or function. cDNAs coding for unknown proteins or predicted genes are not listed.