Overexpression of Munc18-2, open-syntaxin, and SNAP-25 do not affect the transcription level of unrelated proteins but overexpression of Munc18-2 increased the transcription of syntaxin
| Overexpressed protein | Tested mRNA level normalized to the endogenous concentration | |||||
|---|---|---|---|---|---|---|
| Munc18 | Syntaxin | SNAP-25 | Rab3 | Synaptotagmin | Complexin | |
| Munc18-2 | 6.8 | 23.3 | 0.5 | 1.5 | 0.9 | 1.48 |
| Open-syntaxin | 2.5 | >5000 | 0.2 | 1.0 | 1.0 | 0.6 |
| SNAP-25 | 0.8 | 1.1 | >5000 | 0.8 | 0.9 | 0.6 |
The results are expressed as fold increase over endogenous expression level. We detected mRNA of Munc18-1 and -2 but they were pooled together since both isoforms share most features.