Composition of intracellular solutions (in mm)
| Flash and depolarization | Solution containing high [Ca2+]i | |||
|---|---|---|---|---|
| 1 μm | 6 μm | 15 μm | ||
| CaCl2 | 4.5 | 5 | 5 | 5 |
| Ca2+ buffer | 5 (NP-EGTA) | 5.9 (EGTA) | 9 (HEDTA) | 20 (DPTA) |
| Fura-2 | 0.1 | — | — | — |
| Fura-FF | 0.1 | — | — | — |
| Mg-ATP | 2 | 2 | 2 | 2 |
| Na2-GTP | 0.3 | 0.3 | 0.3 | 0.3 |
| Cs-glutamate | 98 | 120 | 110 | 90 |
| HEPES | 40 | 10 | 10 | 10 |
| pH | 7.3 | 7.3 | 7.2 | 7.4 |
NP-EGTA, 5 nitrophenyl-EGTA.