Pharmacological modulation of cytosolic calcium dynamics
| Duration | SOCE (2 mm Ca2+) | Peak F/F0 (1 μm TG) | |||
|---|---|---|---|---|---|
| WT | G93A | WT | G93A | ||
| No pretreatment | 0 | 1.29 ± 0.12 (35) | 1.83 ± 0.16* (38) | 2.07 ± 0.22 (19) | 3.04 ± 0.24* (37) |
| 100 μm 2-APB | 10 min | 0.09 ± 0.04 (19) | 0.65 ± 0.15* (21) | n.d. | n.d. |
| 50 μm ML-9 | 10 min | 0.88 ± 0.12 (30) | 0.60 ± 0.08 (20) | n.d. | n.d. |
| 100 μm BSO | 4 h | 3.06 ± 0.47 (14) | 3.01 ± 0.74 (14) | 5.37 ± 0.67 (14) | 3.82 ± 0.71 (14) |
| 1 mm GSHEE | 4 h | 1.67 ± 0.36 (14) | 1.32 ± 0.35 (16) | n.d. | n.d. |
SOCE defined as the difference between calcium influx peak (F/F0) and baseline during ER calcium depletion by TG (1 μm).
↵*p < 0.05 by Student's t test, G93A SOD1 versus WT astrocytes. Error indicates SEM. Number of astrocytes analyzed per condition is indicated in parentheses. Astrocytes from at least four coverslips from at least three independent spinal cord preparations were analyzed. n.d., Not determined.