Analysis of locomotor activity in transgenic larvae without heatshock
| Hypocretin | Day 5 | ||
|---|---|---|---|
| Wild type (n = 11) | Hypocretin (n = 19) | p values | |
| Waking activity (s/waking min) | 3.54 ± 0.544 | 5.26 ± 0.543 | 3.69E-02 |
| Rest (min/10 min) | 3.10 ± 0.911 | 1.23 ± 0.376 | 7.39E-02 |
| Maximum amplitude/movement (pixels) | 11.60 ± 3.322 | 12.30 ± 1.318 | 2.20E-01 |
| Movement duration (s) | 0.14 ± 0.007 | 0.14 ± 0.003 | 4.01E-01 |
| Movement frequency (Hz) | 0.41 ± 0.080 | 0.61 ± 0.062 | 5.55E-02 |
| Bout duration (s) | 1.49 ± 0.239 | 1.59 ± 0.125 | 5.61E-01 |
| Bout frequency (s) | 0.16 ± 0.035 | 0.19 ± 0.018 | 5.19E-01 |
| Rest latency (1 min) | 11.07 ± 1.25 | 15.96 ± 3.28 | 8.13E-01 |
| Adcyap1b | Wild type (n = 21) | Adcyap1b (n = 26) | p values |
|---|---|---|---|
| Waking activity (s/waking min) | 3.28 ± 0.316 | 2.93 ± 0.239 | 4.16E-01 |
| Rest (min/10 min) | 1.71 ± 0.382 | 2.71 ± 0.426 | 2.19E-01 |
| Maximum amplitude/movement (pixels) | 9.80 ± 1.584 | 9.58 ± 1.618 | 8.47E-01 |
| Movement duration (s) | 0.13 ± 0.003 | 0.13 ± 0.003 | 6.38E-01 |
| Movement frequency (Hz) | 0.41 ± 0.039 | 0.36 ± 0.032 | 3.04E-01 |
| Bout duration (s) | 1.07 ± 0.113 | 1.05 ± 0.108 | 9.83E-01 |
| Bout frequency (s) | 0.19 ± 0.019 | 0.18 ± 0.021 | 4.94E-01 |
| Rest latency (1 min) | 9.15 ± 2.42 | 8.16 ± 0.98 | 9.91E-01 |
| Cart | Wild type (n = 20) | Cart (n = 26) | p values |
|---|---|---|---|
| Waking activity (s/waking min) | 3.79 ± 0.368 | 3.38 ± 0.358 | 3.80E-01 |
| Rest (min/10 min) | 1.52 ± 0.323 | 1.93 ± 0.510 | 5.54E-01 |
| Maximum amplitude/movement (pixels) | 12.15 ± 2.952 | 11.68 ± 3.197 | 5.99E-01 |
| Movement duration (s) | 0.14 ± 0.004 | 0.14 ± 0.005 | 8.26E-01 |
| Movement frequency (Hz) | 0.43 ± 0.038 | 0.38 ± 0.046 | 4.30E-01 |
| Bout duration (s) | 1.03 ± 0.104 | 0.99 ± 0.077 | 9.30E-01 |
| Bout frequency (s) | 0.19 ± 0.020 | 0.17 ± 0.020 | 5.99E-01 |
| Rest latency (1 min) | 38.32 ± 14.55 | 36.64 ± 20.77 | 7.09E-01 |
| Cck | Wild type (n = 29) | Cck (n = 17) | p values |
|---|---|---|---|
| Waking activity (s/waking min) | 4.50 ± 0.362 | 3.90 ± 0.257 | 4.19E-01 |
| Rest (min/10 min) | 1.26 ± 0.261 | 2.20 ± 0.467 | 9.60E-02 |
| Maximum amplitude/movement (pixels) | 13.29 ± 2.039 | 13.22 ± 2.983 | 9.73E-01 |
| Movement duration (s) | 0.15 ± 0.003 | 0.15 ± 0.005 | 6.41E-01 |
| Movement frequency (Hz) | 0.49 ± 0.041 | 0.42 ± 0.033 | 3.57E-01 |
| Bout duration (s) | 1.60 ± 0.241 | 1.57 ± 0.258 | 9.73E-01 |
| Bout frequency (s) | 0.17 ± 0.012 | 0.16 ± 0.018 | 6.74E-01 |
| Rest latency (1 min) | 10.19 ± 1.65 | 13.96 ± 3.13 | 7.97E-02 |
| Cgrp | Wild type (n = 40) | Cgrp (n = 43) | p values |
|---|---|---|---|
| Waking activity (s/waking min) | 3.46 ± 0.276 | 4.12 ± 0.278 | 5.56E-02 |
| Rest (min/10 min) | 2.48 ± 0.403 | 1.64 ± 0.337 | 7.54E-02 |
| Maximum amplitude/movement (pixels) | 11.00 ± 1.680 | 12.18 ± 1.596 | 1.11E-01 |
| Movement duration (s) | 0.14 ± 0.003 | 0.14 ± 0.003 | 1.96E-01 |
| Movement frequency (Hz) | 0.40 ± 0.035 | 0.48 ± 0.033 | 3.77E-02 |
| Bout duration (s) | 1.32 ± 0.138 | 1.11 ± 0.077 | 6.95E-01 |
| Bout frequency (s) | 0.18 ± 0.018 | 0.22 ± 0.016 | 9.01E-02 |
| Rest latency (1 min) | 47.50 ± 11.14 | 45.03 ± 9.52 | 8.26E-01 |
| Galanin | Wild type (n = 19) | Galanin (n = 35) | p values |
|---|---|---|---|
| Waking activity (s/waking min) | 3.79 ± 0.368 | 3.38 ± 0.358 | 3.80E-01 |
| Rest (min/10 min) | 1.52 ± 0.323 | 1.93 ± 0.510 | 5.54E-01 |
| Maximum amplitude/movement (pixels) | 12.15 ± 2.952 | 11.68 ± 3.197 | 5.99E-01 |
| Movement duration (s) | 0.14 ± 0.004 | 0.14 ± 0.005 | 8.26E-01 |
| Movement frequency (Hz) | 0.43 ± 0.038 | 0.38 ± 0.046 | 4.30E-01 |
| Bout duration (s) | 1.03 ± 0.104 | 0.99 ± 0.077 | 9.30E-01 |
| Bout frequency (s) | 0.19 ± 0.020 | 0.17 ± 0.020 | 5.99E-01 |
| Rest latency (1 min) | 9.20 ± 0.76 | 11.66 ± 1.86 | 5.86E-01 |
| Nociceptin | Wild type (n = 44) | Nociceptin (n = 44) | |
|---|---|---|---|
| Waking activity (s/waking min) | 2.97 ± 0.232 | 2.78 ± 0.207 | 6.22E-01 |
| Rest (min/10 min) | 2.83 ± 0.388 | 3.69 ± 0.323 | 4.01E-02 |
| Maximum amplitude/movement (pixels) | 9.57 ± 1.344 | 9.74 ± 1.146 | 4.53E-01 |
| Movement duration (s) | 0.14 ± 0.003 | 0.14 ± 0.003 | 1.76E-01 |
| Nociceptin | Day 5 | ||
|---|---|---|---|
| Wild type (n = 11) | Hypocretin (n = 19) | p values | |
| Movement frequency (Hz) | 0.33 ± 0.031 | 0.27 ± 0.026 | 1.56E-01 |
| Bout duration (s) | 0.94 ± 0.074 | 1.23 ± 0.097 | 1.12E-02 |
| Bout frequency (s) | 0.17 ± 0.015 | 0.12 ± 0.012 | 1.41E-02 |
| Rest latency (1 min) | 6.40 ± 0.44 | 6.24 ± 0.42 | 6.89E-01 |
Values are mean ± SEM p values from Kruskall–Wallis one-way ANOVA. Larvae with the HS-nociceptin transgene exhibited decreases in measures of spontaneous locomotion independent of heatshock, but these decreases were not statistically significant after correction for multiple comparisons.