PCR conditions, slope, correlation coefficient (R2) and efficiency values (E) for each primer
| Gene (Consensus Coding Sequence identification number) | Sequence | Product length (bp) | Annealing (°C) | Slope | R2 | E |
|---|---|---|---|---|---|---|
| Olf16 (15521.1) | F: 5′-CTTGTTGGTTTGTGGGTCTTTAGG-3′; R: 5′-CAAAAGGGCAAATGGAACATG-3′ | 80 | 60 | −3.33 | 0.99 | 1.99 |
| Ac3 (49017.1) | F: 5′-AGGCGACTGTTACTACTGCATCTG-3′; R: 5′-ACGTACGAGATGGCCTCTACCA-3′ | 109 | 65 | −3.4 | 0.99 | 1.96 |
| Cnga2 (30184.1) | F: 5′-GGCACCAAAAAGAAATTTGAACTG-3′; R: 5′-CAGGCTCTGGCTACCAACAGA-3′ | 101 | 62 | −3.28 | 0.99 | 2.01 |
| Pde1c (51784.1) | F: 5′-GTCCCAGCGTCGTGATTAGC-3′; R: 5′-TCATGACATCTCGAGCAAGTCTTT-3′ | 66 | 60 | −3.26 | 0.99 | 1.99 |
| Gapdh (51913.1) | F: 5′-CCTGGAGAAACCTGCCAAGTAT-3′; R: 5′-CTGTTGAAGTCGCAGGAGACAA-3′ | 77 | 62 | |||
| Suclg (27271.1) | F: 5′-GCGTCATCAACCCTGGAGAA-3′; R: 5′-CTGCCCCAATCCGACTTG-3′ | 139 | 60 | −3.2 | 0.99 | 2.05 |