Table 1.

Arp2∕3 role in axon outgrowth rate and length

LamininL1
Axon outgrowth ratea (μm∕h)
    DMSO20.68 ± 0.588115.26 ± 0.8684
    CK66618.02 ± 0.802410.76 ± 0.5519
Axon lengthb (μm)
    DMSO235.2 ± 11.88198.2 ± 11.10
    CK666195.6 ± 12.61153.0 ± 9.936
    EGFP236.8 ± 11.21239.5 ± 13.39
    EGFP-CA182.2 ± 11.66144.1 ± 12.01
  • aMeasurements were done from time-lapse videos of E7 DRG explants' growth cones on L1 treated with DMSO or 50M CK666 4 h prior to imaging.

  • bMeasurements were done on dissociated low-density cultures on L1 from E7 DRG neurons grown for 24 h, fixed, and stained with antibodies against neuronal β3-tubulin. For pharmacological inhibition of Arp2∕3 with CK666, neurons were allowed to adhere for 6 h before adding the drug.