Real-time PCR primer sequences
| Gene | Primer name | Primer sequence | Primer range | Amplimer size (bp) |
|---|---|---|---|---|
| Gabra1 | Gabra1-F | GTCCTCTGCACTGAGAATCGC | 1620–1700 | 81 |
| Gabra1-R | CGAATTCTTTTAAGACAGAGGCAGTA | |||
| Gabra2 | Gabra2-F | TCATTTTTGCTTTGTACAGTCTGACT | 1475–1588 | 114 |
| Gabra2-R | GCAAGTGCAGGTCTCCTTTAGAG | |||
| Gabra3 | Gabra3-F | ATTTCCCGCATCATCTTCCC | 1612–1696 | 85 |
| Gabra3-R | TGATAGCGGATTCCCTGTTCAC | |||
| GPHN | GPHN-F | ACCTCTGGGCATGCTCTCTA | 512–610 | 99 |
| GPHN-R | TGAAAGCATTCCTGAGATCC | |||
| DAG1 | DAG1-F | GAATCCGCACTACCACCAGT | 1549–1640 | 92 |
| DAG1-R | CCAGGCATCAACCCTGTCGA | |||
| NLGN2 | NLGN2-F | CAGAAGGGCTGTTCCAGAAG | 1389–1490 | 102 |
| NLGN2-R | TGGCTGCCAGCAGCCGCGTG | |||
| ARHGEF9 | ARHGEF9-F | CCACCTCAGCGAGATAGGAC | 366–465 | 100 |
| ARHGEF9-R | GAGCTCCATGCAGGCATCCA | |||
| TFRC | TFRC-F | CGGCTACCTGGGCTATTGTA | 246–330 | 85 |
| TFRC-R | TTCTGACTTGTCCGCCTCTT |
Primer range numbering is based on the CDS sequence.