Data shown are latencies to intromission and ejaculation (seconds) from control experiments conducted to determine that the effects of MOR blockade on loss of long-term reinforcement of sexual behavior occurred independent from a lack of naloxone administration on the final mating test day
| Experiment | Day | Saline during final mating test | Naloxone during final mating test | ||
|---|---|---|---|---|---|
| Intromission latency | Ejaculation latency | Intromission latency | Ejaculation latency | ||
| NLX or saline administration on final mating test day: 7 d after mating experience (Fig. 5A) | 1 | 157.7 ± 75.0 | 707.0 ± 501.1 | 345.6 ± 139.4 | 645.7 ± 124.8 |
| 5 | 25.8 ± 8.9* | 341.3 ± 83.5 | 74.6 ± 22.5* | 404.7 ± 83.19 | |
| Test | 101.2 ± 27.1# | 580.2 ± 151.0 | 161.9 ± 35.1# | 711.3 ± 136.9 | |
| NLX or saline administration on final mating test day: 1 d after mating experience (Fig. 5E) | 1 | 394.3 ± 110 | 748.3 ± 243 | 161.5 ± 58.2 | 286.5 ± 66.8 |
| 5 | 61.6 ± 22.0* | 418.0 ± 150 | 38.3 ± 16.4* | 365.25 ± 77.9 | |
| Test | 60.8 ± 15.5* | 265.2 ± 37.8 | 39.3 ± 13.7* | 340.5 ± 73.1 | |
Data presented are for behavior on days 1 and 5 of five consecutive days of mating and the final mating test, either 1 d (saline, n = 5; naloxone, n = 4) or 7 d (saline, n = 6; naloxone, n = 7) after the fifth mating session. Data are presented as mean ± SEM;
↵* indicates significant difference compared with day 1 within treatment; # indicates significant difference between test day and day 5 within treatment.