Table 2.

Time constants for the exocytosis and endocytosis associated with the different response profiles after brief stimulation

Profiles	Time to reach half-maximal response (τ, s)			Time recovery constant (τ, s)
Profiles	Control	KT5823	p	Control	KT5823	p
1	3.76 ± 0.07	4.19 ± 0.08	>0.05	12.30 ± 0.83	7.45 ± 0.44^*	<0.00001
2	4.75 ± 0.04	4.42 ± 0.04	>0.05	19.20 ± 1.10	14.69 ± 0.68^*	0.027342
3	6.08 ± 0.14	5.77 ± 0.09	>0.05	10.95 ± 1.04	9.42 ± 0.76	0.306624
4	4.07 ± 0.15	2.24 ± 0.73	>0.05	13.29 ± 0.41	11.01 ± 0.27^*	<0.00001
5	3.63 ± 0.17	3.60 ± 0.07	>0.05	15.07 ± 0.89	10.58 ± 0.63^*	0.000855

The cells were stimulated with 50 mm KCl for 10 s and the increase in fluorescence was adjusted to (F) = F_max*t/(τ + t), where τ is the time necessary to reach half-maximal fluorescence. The fluorescence decay was adjusted to F = F₀+Ae^−t/τ, where τ is the time constant for the basal recovery. Data are mean ± SEM from individual experiments control (control, n = 10; KT-5823-treated cells, n = 8).
↵* represents significance between the control and the KT5823-treated cells.