Quantification of song degradation
| No. of days | LD+T 21 | SD | ANOVA | |||||
|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 4 | 7 | 14 | 28 | |||
| Song rate | (n = 18 birds) | (n = 10) | (n = 8) | (n = 8) | (n = 8) | (n = 9) | (n = 8) | |
| Max no. songs/hour | 152 ± 33b | 73 ± 29a,b | 93 ± 46a,b | 104 ± 46a,b | 32 ± 9b | 33 ± 21b | 17 ± 12b | F(6, 62)=2.5623, p = 0.0279 |
| Spectral feature | (n = 11 birds*) | (n = 8) | (n = 4) | (n = 6) | (n = 7) | (n = 4) | (n = 2) | |
| Duration (s) | 1.68 ± 0.07b | 1.65 ± 0.09b | 1.42 ± 0.14a,b | 1.16 ± 0.19b | 1.16 ± 0.09b | 1.33 ± 0.18a,b | 1.09 ± 0.31b | F(6, 38)=3.9942, p = 0.0042 |
| Aggregate entropy | 3.51 ± 0.11b | 3.48 ± 0.14a,b | 3.27 ± 0.12a,b,c | 3.07 ± 0.14b,c | 3.14 ± 0.16b,c | 3.37 ± 0.16a,b | 2.63 ± 0.02c | F(6, 38)=2.5117, p = 0.0381 |
| Average power (dB) | 64.60 ± 1.89a,b | 66.01 ± 3.38a,b | 61.06 ± 6.83b | 64.64 ± 6.05a,b | 71.35 ± 4.84a,b | 75.72 ± 3.43b | 56.70 ± 6.59b | F(6, 38)=1.4236, p = 0.2311 |
| Coefficient of variation | ||||||||
| High frequency | 0.05 ± 0.01b | 0.05 ± 0.02b | 0.09 ± 0.05b | 0.08 ± 0.02b | 0.08 ± 0.02b | 0.05 ± 0.02b | 0.19 ± 0.05b | F(6, 38)=2.4167, p = 0.0446 |
| Low frequency | 0.12 ± 0.02 | 0.14 ± 0.05 | 0.23 ± 0.05 | 0.12 ± 0.03 | 0.11 ± 0.03 | 0.08 ± 0.04 | 0.08 ± 0.04 | F(6, 38)=0.2938, p = 0.9361 |
| Cross correlation | ||||||||
| To LD+T 21 d | 21 d: 0.48 ± 0.02b 23 d: 0.43 ± 0.02a,b | 0.36 ± 0.04b,c | 0.37 ± 0.02b,c | 0.37 ± 0.04b,c | 0.34 ± 0.04c | 0.32 ± 0.03c | N/A | F(6, 62)=4.0390, p = 0.0018 |
| Percentage complete | ||||||||
| Relative to LD+T | 0.943 ± 0.04b | 0.89 ± 0.06a,b | 0.64 ± 0.07c | 0.71 ± 0.04c | 0.87 ± 0.09a,b | 0.73 ± 0.02b,c | F(6, 35)=9.8902, p < 0.001 | |
Bold indicates significant differences.
↵*For spectral features, CV, cross correlation, and percentage complete, only birds that sang at least 30 songs for the given day were analyzed. All values are mean ± SEM.
↵a,benotes significant differences across treatment groups. Lack of letters indicates no differences.