Injection sites (sites containing eYFP-positive cell bodies) and fiber trajectories per animal
| Case | Injection spot | Distance (mm) behind bregma | Medial route (route 1) | Septal route (route 2) | Internal capsule route (route 3) | Lateral route (route 4) | eYFP-positive fibers in the anterior commissure | eYFP-positive fibers in the fornix | eYFP-positive fibers in the stria terminalis |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Anterior HDB | −0.26 | ++ | ++ | − | − | − | + | + |
| 2 | Anterior HDB | 0.02 | ++ | + | − | − | − | + | + |
| 3 | Mid/posterior HDB | 0.02 | ++ | + | − | + | + | + | + |
| 4 | Mid HDB/VP/MCPO | 0.22 | + | + | + | + | − | ++ | ++ |
| 5 | VP/NB | 0.1 | + | + | + | + | − | − | ++ |
| 6 | BNST/NB | 0.1 | − | − | ++ | + | − | − | +++ |
| 7 | NB | 0.34 | − | − | + | ++ | + | − | + |
| 8 | SI/ZI | 0.7 | − | − | + | ++ | − | − | + |
| 9 | NB | 0.82 | − | − | +++ | ++ | + | − | ++ |
We identified four main routes taken by YFP-positive fibers from their parent cell bodies to mPFC targets: medial, septal, internal capsule, and lateral. ZI, Zona incerta.