Primer sequences for α-adrenergic receptors and GAD
| Primer | Sequence (5′-3′) | PCR product (bp) |
|---|---|---|
| Adrenergic α1A | ||
| Outside | ||
| Forward | GAG CAA GTG ACG CTC CGT AT | 348 |
| Reverse | ACA CTG GAT TCG CAG CAC AT | |
| Nested | ||
| Forward | ATT GTG GTG GGA TGC TTC GT | 95 |
| Reverse | ACT GTT TCC GGT GGC TTG AA | |
| Adrenergic α1B | ||
| Outside | ||
| Forward | TTT CAT GAG GAC ACC CTC AGC AGT | 303 |
| Reverse | CAT GAA GGC GCG TTT GAA CTC CTT | |
| Nested | ||
| Forward | CCT TGG GCA TTG TAG TCG GAA TGT | 136 |
| Reverse | TTG AAG TAG CCC AGC CAG AAC ACT | |
| Adrenergic α1D | ||
| Outside | ||
| Forward | TCC GTA AGG CTG CTC AAG TT | 176 |
| Reverse | TAG CCC AGC CAG AAG ATG AC | |
| Nested | ||
| Forward | CAA GAC CCT AGC CAT CGT CG | 112 |
| Reverse | GAA GAC GCC CTC TGA TGG TT | |
| Adrenergic α2A | ||
| Outside | ||
| Forward | TGG CCA ACG AGG TTA TGG GTT ACT | 210 |
| Reverse | AGA TGA CCC ACA CGG TGA CAA TGA | |
| Nested | ||
| Forward | TCT ATT TGG CTC TCG ACG TGC TCT | 115 |
| Reverse | GTG CGC TTC AGG TTG TAC TCG AT | |
| Adrenergic α2B | ||
| Outside | ||
| Forward | TCT TGG CTT CCA GCA TCG GAT CTT | 457 |
| Reverse | AAG GGT GGG TTG AAT ACT GAG GCA | |
| Nested | ||
| Forward | TGT AAT TGC CAA ACG CAG CCA CTG | 162 |
| Reverse | TGT CCA TTA GCC TCT CCG ACA GAA | |
| Adrenergic α2C | ||
| Outside | ||
| Forward | AAG TGT GGT GTG GTG TAT ACC TGG | 291 |
| Reverse | AGC CTA TGC AGG AGG ACA AGA TGT | |
| Nested | ||
| Forward | ATC GTG CAC CTG TGT GCC ATT AGT | 139 |
| Reverse | GGA AGG AGA TGA CAG CGG AGA TGA | |
| GAD1 | ||
| Outside | ||
| Forward | ACA ACC TTT GGC TGC ATG TGG ATG | 331 |
| Reverse | AAT CCC ACG GTG CCC TTT GCT TTC | |
| Nested | ||
| Forward | AGT CAC CTG GAA CCC TC | 128 |
| Reverse | GCT TGT CTG GCT GGA A |