Primer description and sequences
| Gene | Primer sequence 5′ → 3′ | Function | |
|---|---|---|---|
| Forward | Reverse | ||
| β-actin | TTCCTTCCTGGGTATGGAAT | GAGGAGCAATGATCTTGATC | Cytoskeletal protein (housekeeping gene) |
| CD163 | TCATTCAACCCTCACTGCAC | CTTGAGGAAACTGTAAGCCG | Macrophage antigen not expressed by microglia |
| GFAP | AGATCCGAGAAACCAGCCTG | CCTTAATGACCTCGCCATCC | Astrocyte antigen |
| IL-1β | CCTTGTGCAAGTGTCTGAAG | GGGCTTGGAAGCAATCCTTA | Proinflammatory cytokine |
| IL-6 | AGAAAAGAGTTGTGCAATGGCA | GGCAAATTTCCTGGTTATATCC | Proinflammatory cytokine |
| MHCII | AGCACTGGGAGTTTGAAGAG | AAGCCATCACCTCCTGGTAT | Microglia/macrophage antigen |
| IκBα | CACCAACTACAACGGCCACA | GCTCCTGAGCGTTGACATCA | Induced by NF-κB to inhibit NF-κB function |
| TNFα | CAAGGAGGAGAAGTTCCCA | TTGGTGGTTTGCTACGACG | Proinflammatory cytokine |