Summary of synaptic response from different recording conditions at P16–P19 calyx of Held synapsea
| Parameters | Conditions | Stimulation | Paired t test | ||
|---|---|---|---|---|---|
| Dep1 ms (mean ± SEM; n) | Dep10 ms (mean ± SEM; n) | Dep50 ms (mean ± SEM; n) | p value (Dep1 ms vs Dep 10 ms) | ||
| EPSC amplitude (nA) | 2 mm [Ca2+]e and 0.5 mm [EGTA]i (a) | 22.0 ± 2.40 (11) | 21.00 ± 2.20 (11) | NA | p > 0.05 |
| 2 mm [Ca2+]e and 5 mm [EGTA]i (b) | 21.0 ± 1.83 (13) | 20.3 ± 1.73 (13) | NA | p > 0.05 | |
| 2 mm [Ca2+]e and 20 mm [EGTA]i (c) | 4.73 ± 1.03 (8) | 5.28 ± 0.56 (8) | 5.02 ± 0.48 (7) | p > 0.05 [1 ms vs 10 ms]; p > 0.05 [1 ms vs 50 ms]; p > 0.05 [10 ms vs 50 ms] | |
| 1.2 mm [Ca2+]e and 0.5 mm [EGTA]i (d) | 17.1 ± 3.07 (9) | 18.2 ± 3.33 (9) | NA | p > 0.05 | |
| Unpaired t test | Compare EGTA effect | p > 0.05, a versus b; p < 0.0001, a versus c | p > 0.05, a versus b; p < 0.0001, a versus c | ||
| Compare calcium effect | p > 0.05, a versus d | p > 0.05, a versus d | |||
| Calcium influx charge (pC) | 2 mm [Ca2+]e and 0.5 mm [EGTA]i (a) | 1.51 ± 0.11 (11) | 11.2 ± 1.73 (11) | NA | NA |
| 2 mm [Ca2+]e and 5 mm [EGTA]i (b) | 1.66 ± 0.10 (13) | 12.5 ± 0.69 (13) | NA | NA | |
| 2 mm [Ca2+]e and 20 mm [EGTA]i (c) | 1.19 ± 0.17 (8) | 9.62 ± 0.95 (8) | 42.31 ± 4.32 (7) | NA | |
| 1.2 mm [Ca2+]e and 0.5 mm [EGTA]i (d) | 1.21 ± 0.16 (9) | 9.09 ± 0.88 (9) | NA | NA | |
| Unpaired t test | Compare EGTA effect | p > 0.05, a versus b; p > 0.05, a versus c | p > 0.05, a versus b; p > 0.05, a versus c | ||
| Compare calcium effect | p = 0.058, a versus d | p < 0.05, a versus d | |||
| 10%–90% EPSC rise time (ms) | 2 mm [Ca2+]e and 0.5 mm [EGTA]i (a) | 0.61 ± 0.02 (11) | 0.60 ± 0.02 (11) | NA | p > 0.05 |
| 2 mm [Ca2+]e and 5 mm [EGTA]i (b) | 0.65 ± 0.02 (13) | 0.67 ± 0.03 (13) | NA | p > 0.05 | |
| 2 mm [Ca2+]e and 20 mm [EGTA]i (c) | 0.68 ± 0.02 (8) | 1.34 ± 0.19 (8) | 1.47 ± 0.18 (7) | p < 0.05 [1 ms vs 10 ms]; p < 0.05 [1 ms vs 50 ms]; p > 0.05 [10 ms vs 50 ms] | |
| 1.2 mm [Ca2+]e and 0.5 mm [EGTA]i (d) | 0.66 ± 0.01 (9) | 0.75 ± 0.03 (9) | NA | p < 0.05 | |
| Unpaired t test | Compare EGTA effect | p > 0.05, a versus b; p < 0.05, a versus c | p > 0.05, a versus b; p < 0.001, a versus c | ||
| Compare calcium effect | p < 0.05, a versus d | p < 0.001, a versus d | |||
| Synaptic delay (ms) | 2 mm [Ca2+]e and 0.5 mm [EGTA]i (a) | 1.02 ± 0.03 (11) | 1.00 ± 0.02 (11) | NA | p > 0.05 |
| 2 mm [Ca2+]e and 5 mm [EGTA]i (b) | 1.07 ± 0.04 (13) | 1.05 ± 0.04 (13) | NA | p > 0.05 | |
| 2 mm [Ca2+]e and 20 mm [EGTA]i (c) | 1.34 ± 0.06 (8) | 1.52 ± 0.12 (8) | 1.49 ± 0.14 (7) | p > 0.05 [1 ms vs 10 ms ]; p > 0.05 [1 ms vs 50 ms ]; p > 0.05 [10 ms vs 50 ms ] | |
| 1.2 mm [Ca2+]e and 0.5 mm [EGTA]i (d) | 1.14 ± 0.03 (9) | 1.13 ± 0.02 (9) | NA | p > 0.05 | |
| Unpaired t test | Compare EGTA effect | p > 0.05, a versus b; p < 0.0001, a versus c | p > 0.05, a versus b; p < 0.0001, a versus c | ||
| Compare calcium effect | p < 0.01, a versus d | p < 0.001, a versus d | |||
↵aTwo-tailed unpaired t test was used to compare a versus b, a versus c; one-tailed unpaired t test was used to compare a versus d. NA, Not analyzed.