Parameters | Conditions | Stimulation | Paired t test | ||
---|---|---|---|---|---|
Dep1 ms (mean ± SEM; n) | Dep10 ms (mean ± SEM; n) | Dep50 ms (mean ± SEM; n) | p value (Dep1 ms vs Dep10 ms) | ||
Peak release rate (vesicles/ms) | 2 mm [Ca2+]e and 0.5 mm [EGTA]i (a) | 1605 ± 160 (11) | 1541 ± 154 (11) | NA | p > 0.05 |
2 mm [Ca2+]e and 5 mm [EGTA]i (b) | 1559 ± 140 (13) | 1502 ± 138 (13) | NA | p > 0.05 | |
2 mm [Ca2+]e and 20 mm [EGTA]i (c) | 335 ± 73 (8) | 313 ± 51 (8) | 260 ± 43 (7) | p > 0.05 [1 ms vs 10 ms]; p > 0.05 [1 ms vs 50 ms]; p > 0.05 [10 ms vs 50 ms] | |
1.2 mm [Ca2+]e and 0.5 mm [EGTA]i (d) | 1123 ± 215 (8) | 1179 ± 240 (8) | NA | p > 0.05 | |
Unpaired t test | Compared EGTA effect | p > 0.05, a versus b; p < 0.0001, a versus c | p > 0.05, a versus b; p < 0.0001, a versus c | p > 0.05, a versus b; p < 0.0001, a versus c | |
Compared calcium effect | p > 0.05, a versus d | p > 0.05, a versus d | p < 0.05, a versus d | ||
Vesicles released (vesicles) | 2 mm [Ca2+]eand 0.5 mm [EGTA]i (a) | 1759 ± 183 (11) | 2714 ± 247 (11) | NA | NA |
2 mm [Ca2+]e and 5 mm [EGTA]i (b) | 1769 ± 181 (13) | 3031 ± 295 (13) | NA | NA | |
2 mm [Ca2+]e and 20 mm [EGTA]i (c) | 438 ± 93 (8) | 1204 ± 124 (8) | 1748 ± 176 (7) | p < 0.0001 [1 ms vs 10 ms]; p < 0.0001 [1 ms vs 50 ms]; p < 0.001 [10 ms vs 50 ms] | |
1.2 mm [Ca2+]e and 0.5 mm [EGTA]i (d) | 1188 ± 223 (8) | 2635 ± 530 (8) | NA | NA | |
Unpaired t test | Compared EGTA effect | p > 0.05, a versus b; p < 0.0001, a versus c | p > 0.05, a versus b; p < 0.0001, a versus c | ||
Compared calcium effect | p < 0.05, a versus d | p > 0.05, a versus d |
↵aTwo-tailed unpaired t test was used to compare a versus b, a versus c; one-tailed unpaired t test was used to compare a versus d. NA, Not analyzed.