Table 1.

Multivariate ANOVA main effects, interactions, and p values for c-Fos counts in ad libitum-fed or DEP rats following NH, RES, or EP treatment

Effect source	Dependent variable	F	p value
Treatment (F_(2,31))	Activation of GLP-1 neurons (%)	43.52	<0.001^*
NH vs RES vs EP	Activation of PrRP⁺ A2 neurons (%)	36.79	<0.001^*
	Activation of PrRP⁻ A2 neurons (%)	45.06	<0.001^*
	cFos⁺ Neurons in mpPVN	21.09	<0.001^*
	cFos⁺ Neurons per 100 μm² in vlBST	71.25	<0.001^*
	Number of GLP-1 neurons per section	0.05	>0.05
	Number of PrRP neurons per section	2.96	>0.05
Feeding status (F_(1,31))	Activation of GLP-1 neurons (%)	800.97	<0.001^*
Ad libitum vs DEP	Activation of PrRP⁺ A2 neurons (%)	175.87	<0.001^*
	Activation of PrRP⁻ A2 neurons (%)	29.80	<0.001^*
	cFos⁺ neurons in mpPVN	3.51	0.07
	cFos⁺ neurons per 100 μm² in vlBST	16.89	<0.001^*
	Number of GLP-1 neurons per section	0.02	>0.05
	Number of PrRP neurons per section	0.89	>0.05
Interaction (F_(2,31))	Activation of GLP-1 neurons (%)	41.79	<0.001^*
Treatment × feeding status	Activation of PrRP⁺ A2 neurons (%)	10.88	<0.001^*
	Activation of PrRP⁻ A2 neurons (%)	6.45	<0.01^*
	cFos⁺ neurons in mpPVN	2.50	>0.05
	cFos⁺ neurons per 100 μm² in vlBST	3.85	<0.05^*
	Number of GLP-1 neurons per section	0.28	>0.05
	Number of PrRP neurons per section	0.26	>0.05