Morphometric analysis of synaptic ultrastructure
| Genotype | Non-Tg control mice (n = 61, N = 3) | UbG76V-GFP-Syb2 mice (n = 55, N = 5) |
|---|---|---|
| Nerve terminal area (μm2) | 2.92 ± 0.29 | 3.44 ± 0.48 |
| Nerve terminal perimeter (μm) | 6.76 ± 0.34 | 7.81 ± 0.63 |
| Presynaptic contact length (μm) | 3.35 ± 0.21 | 2.93 ± 0.32 |
| Presynaptic/perimeter ratio (%) | 48.0 ± 1.3 | 35.3 ± 2.0 * |
| Synaptic vesicle number (per nerve terminal) | 175 ± 14.6 | 120 ± 17.9 † |
| Synaptic vesicle density (per μm2) | 67.6 ± 4.1 | 41.7 ± 3.9 ‡ |
Values are reported as the mean ± SEM. Synaptic contact ratio and synaptic vesicle density were significantly decreased in UbG76V-GFP-Syb2 mice (8 months of age) compared with littermate control mice. n, Number of nerve terminals; N, number of mice.
↵*p = 4.0753 × 10−7.
↵†p = 0.0177.
↵‡p = 1.5886 × 10−5.