Binding of selected ligands to brain membranes derived from NOP+/+, NOP+/eGFP, and NOPeGFP/eGFP mice
| NOP+/+ | NOP+/eGFP | NOPeGFP/eGFP | ||||
|---|---|---|---|---|---|---|
| Ki nm | Hill | Ki nm | Hill | Ki nm | Hill | |
| N/OFQ | 0.17 ± 0.05 | 1.1 ± 0.01 | 0.24 ± 0.1 | 1.07 ± 0.06 | 0.30 ± 0.04 | 1.1 ± 0.06 |
| SB612111 | 4.81 ± 0.68 | 0.85 ± 0.02 | 4.63 ± 0.9 | 0.80 ± 0.04 | 7.27 ± 1.2 | 0.8 ± 0.01 |
| SR16835 | 51.3 ± 17 | 0.97 ± 0.03 | 37.1 ± 1.1 | 0.81 ± 0.06 | 50.8 ± 3.0 | 0.72 ± 0.01 |
Binding to brain membranes from mice of each genotype, as well as data analysis were conducted as described in Materials and Methods. Data presented are from two experiments each conducted in triplicate. Values shown are average ± SD.