Quantification of 5-HT and non-5-HT neurons expressing flippase under control of a Trh promotera
| 5-HT clusters | Trh-FIF (II), FLP reporter | Trh-FIF (III), FLP reporter |
|---|---|---|
| ALP | 1 ± 1 | 0 ± 0 |
| AMP | 1 ± 1 | 0 ± 0 |
| ADMP | 0 ± 0 | 0 ± 0 |
| LP | 8 ± 4 | 7 ± 2 |
| SEL | 7 ± 3 | 7 ± 4 |
| SEM | 5 ± 0 | 5 ± 1 |
| PLP | 3 ± 1 | 4 ± 0 |
| PMPD | 3 ± 1 | 4 ± 2 |
| PMPM | 2 ± 0 | 2 ± 1 |
| PMPV | 5 ± 1 | 9 ± 1 |
| Σ 5-HT neurons | 33 ± 1 | 36 ± 7 |
| Non-5-HT neurons | 11 ± 1 | 8 ± 1 |
↵aThe numbers of 5-HT-positive and 5-HT-negative neurons expressing FIF and their soma cluster designation were immunohistochemically characterized in animals carrying DNA constructs for actin-FRT-Stop-FRT-Gal4, UAS:GFP, and either Trh-FIF located on the second chromosome (II) or third chromosome (III). Anti-GFP immunostaining was used to report flippase activity in combination with anti-5-HT immunostaining. Numbers indicate mean ± SD (n = 3 entire brains).