Primer information
| Sequence | Purpose |
|---|---|
| GGAGTTCCAGCTCCATTGTG | Genotyping for αChnKO mice |
| CAAGGAAAGCCCTCCAGATG | Genotyping for αChnKO mice |
| GTACACTAGGATCTCAGCTC | Genotyping for α1-floxed mice |
| AGCTTGGGTAGTAACAAGAC | Genotyping for α1-floxed mice |
| ATGCAGTAGCACACTTCAGC | Genotyping for α2KO and α2-floxed mice |
| AGTGCTGGCCTTTAAGTATG | Genotyping for α2KO mice |
| AACTATCACACTGTTATGAG | Genotyping for α2-floxed mice |
| ACCTGATGGACATGTTCAGGGATCG | Genotyping Emx1Cre KI-ΔNeo mice |
| TCCGGTTATTCAACTTGCACCATGC | Genotyping Emx1Cre KI-ΔNeo mice |
| GTCGACATGGTGAGCAAGGGCGAGGA | Construction of pK173 |
| GAATTCCTACACCTTCCTCTTCTTCTTGG | Construction of pK173 |
| AGCTGAATTCGCCACCATGAGCGAGCTGATCAAGGAG | Construction of pK165 |
| AGCTGGATCCTCATCTGTGCCCCAGTTTGCTAGG | Construction of pK165 |
| AGTCGACGTCCACCATGGTGCCCAAGA | Construction of pK166 |
| TGGATCCGTCCCCATCCTCGAGCAG | Construction of pK166 |
| AGGATCCGGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACGTGGAGGAGAACCCTGGACCTATGAGCGAGCTGATCA | Construction of pK166 |
| AGAATTCTCATCTGTGCCCCAGTTTGC | Construction of pK166 |
| GACTCTACGGAGTGTCAGGATTTAGTG | Construction of pK193, pK197, and pK195 |
| GACACTCCGTAGAGTCCTTCAGAATT | Construction of pK193, pK197, and pK195 |
| GAGTTTCCCCACACTGAGTG | Titration of AAV |
| GAGGCTTGAGAATGAACCAAGA | Titration of AAV |