Table 2.

List of primary antisera

Target proteinHostDilutionManufacturerCatalog no.RRIDLot
GFPChicken1:2000AvesGFP-1010AB_23073130511FP12
GNAT3Goat1:500Aviva System BiologyOAEB00418AB_10882823G2 E060911
GustducinRabbit1:5000Santa CruzSC-395AB_673678H3112
P2X3Guinea pig1:750NeuromicsGP10108AB_2283325400911
P2X3Human5 μg/mlIntegral Molecularn/an/aLM-021
SerotoninRabbit1:5000Immunostar20080AB_572263924005
  • Both P2X3 antisera were validated by lack of staining of tissue from P2X3-KO mice. LM-021 is a humanized monoclonal antibody directed against the full-length protein using lipoparticle-based immunization (Banik et al., 2011).