Concentration–response analysis reveals that chronic menthol alters α6β2* nAChR stoichiometry
| Treatment condition | |||
|---|---|---|---|
| α6-GFP, β2, β3 no drug | α6-GFPβ2DM no drug | α6-GFP, β2, β3 + menthol | |
| EC50 value (higher sensitivity) | 0.20 ± 0.07 μm | — | 0.17 ± 0.04 μm |
| nh (higher) | 1.01 | — | 0.78 |
| EC50 value (lower sensitivity) | — | 35.9 ± 20.7 μma | 65.0 ± 4.7 μma |
| nh (lower) | — | 0.79 | 2.3 |
| Higher fraction | — | — | 0.43 |
Values are represented as mean ± SEM.
↵aEC50 values are statistically different from α6-GFPβ2β3 nAChRs (no drug). p < 0.005, unpaired t test.