LD activity in clock-cell-specific Lar RNAi knockdown flies does not display defects seen in pdf01 mutants
| Genotype | Total | Evening peak ± SEM | Morning peak ± SEM | Anticipation index ± SEM |
|---|---|---|---|---|
| w1118;UAS-LarRNAi/+;+ | 16 | 12.0 ± 0.08 | 0.6 ± 0.08 | 0.79 ± 0.04 |
| w1118;UAS-LarRNAi/timGal4;+ | 16 | 12.1 ± 0.06† | 0.5 ± 0.00 | 0.95 ± 0.03∧ |
| w1118;UAS-LarRNAi/+;pdfGal4/+ | 14 | 12.3 ± 0.07† | 0.5 ± 0.04 | 0.82 ± 0.06‡ |
| y w;+;pdf01/pdf01 | 14 | 9.8 ± 0.03* | 0.6 ± 0.08 | 0.60 ± 0.06# |
Adult males were analyzed for behavioral rhythms during LD for at least 7 d. The phase of activity peaks and the anticipation of lights-on during LD cycles were analyzed as described (see Materials and Methods). Total, number of flies analyzed; Morning peak ± SEM, Zeitgeber Time of the morning activity peak ± SEM; Evening peak ± SEM, Zeitgeber Time of the evening activity peak ± SEM; Anticipation index ± SEM, the amount of activity that occurs in anticipation of lights-on ± SEM.
↵*Significantly different (p < 0.001) compared to clock-cell-specific Lar RNAi knockdown and control strains.
↵#Significantly different (p < 0.05) compared to clock-cell-specific Lar RNAi knockdown and control strains.
↵†Not different (p ≥ 0.07) than w1118;UAS-LarRNAi/+;+ control.
↵‡Not different (p = 0.73) than w1118;UAS-LarRNAi/+;+ control.
↵∧Significantly better anticipation (p = 0.02) than w1118;UAS-LarRNAi/+;+ control.