Primer sequences for real-time RT-PCR
| Mus gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| 18S | TTCGGAACTGAGGCCATGAT | TTTCGCTCTGGTCCGTCTTG |
| BMP2 | CGTGCGCAGCTTCCATCACG | GAAGAAGCGCCGGGCCGTTT |
| BMP4 | GCATCCGAGCTGAGAGACCCCA | ATCCCATCAGGGACGGAGACCA |
| FGF2 | GGCTGCTGGCTTCTAAGTGT | ACTGGAGTATTTCCGTGACCG |
| ID2 | GCTCTACAACATGAACGACTGCTACT | TGCAGGTCCAAGATGTAATCGA |
| ID4 | GAGACTCACCCTGCTTTGCT | AGAATGCTGTCACCCTGCTT |
| IGF-1 | TGACATGCCCAAGACTCAGAAG | GCGGTGATGTGGCATTTTC |
| IL-1β | CAGGCTCCGAGATGAACAAC | GGTGGAGAGCTTTCAGCTCATAT |
| PDGF-A | ACCCCACATCGGCCAACT | CCGTGAAGGCTGGCACTT |
| TGFβ | TGAGTGGCTGTCTTTTGACGTC | TTCATGTCATGGATGGTGCC |