Shock reactivity and olfactory acuity of flies expressing RNAi-Nep(A) under the control of the MB-Gal80;c316 driver
| Genotype | Shock reactivity | Olfactory acuity | |
|---|---|---|---|
| Octanol | Methylcyclohexanol | ||
| MB-Gal80;c316/+ | 60.80 ± 3.405 | 60.63 ± 6.219 | 60.90 ± 6.667 |
| MB-Gal80;c316/Nep1A | 58.30 ± 1.808 | 67.10 ± 7.114 | 58.70 ± 5.173 |
| +/Nep1A | 64.10 ± 4.927 | 62.10 ± 5.288 | 59.50 ± 5.300 |
| MB-Gal80;c316/+ | 63.25 ± 4.366 | 52.36 ± 7.890 | 65.25 ± 7.345 |
| MB-Gal80;c316/Nep2A | 62.83 ± 3.946 | 47.17 ± 5.455 | 73.13 ± 4.155 |
| +/Nep2A | 77.67 ± 3.530 | 60.15 ± 4.650 | 69.13 ± 8.532 |
| MB-Gal80;c316/+ | 58.43 ± 5.149 | 49.00 ± 4.472 | 54.56 ± 3.986 |
| MB-Gal80;c316/Nep3A | 52.50 ± 4.056 | 54.75 ± 6.648 | 53.42 ± 4.763 |
| +/Nep3A | 73.70 ± 4.261 | 51.17 ± 6.457 | 54.33 ± 3.945 |
| MB-Gal80;c316/+ | 58.57 ± 4.961 | 52.33 ± 4.330 | 58.56 ± 4.628 |
| MB-Gal80;c316/Nep4A | 56.90 ± 8.849 | 52.00 ± 4.424 | 62.00 ± 4.149 |
| +/Nep4A | 58.30 ± 5.863 | 53.08 ± 5.016 | 52.08 ± 3.718 |
Data are shown as means ± SEM. All genotypes tested exhibit normal shock reactivity (Nep1A: F(2,29) = 0.6487, p = 0.5307, n = 10; Nep2A: F(2,35) = 4.544, p = 0.0181, n = 12, post-hoc Newman–Keuls test, MB-Gal80;c316/Nep2A vs +/Nep2A *p < 0.05; Nep3A: F(2,26) = 6.699, p = 0.0049, n ≥ 7, post hoc Newman–Keuls test, MB-Gal80:c316/Nep3A vs +/Nep3A **p < 0.01; Nep4A: F(2,26) = 0.01578, p = 0.9844, n ≥ 7) and normal olfactory acuity for octanol (Nep1A: F(2,27) = 0.2913, p = 0.7498, n ≥ 8; Nep2A: F(2,35) = 1.249, p = 0.3000, n ≥ 11; Nep3A: F(2,32) = 0.2108, p = 0.8111, n ≥ 9; Nep4A: F(2,32) = 0.01515, p = 0.9850, n ≥ 9) and methylcyclohexanol (Nep1A: F(2,29) = 0.03746, p = 0.9633, n = 10; Nep2A: F(2,23) = 0.3230, p = 0.7275, n = 8; Nep3A: F(2,32) = 0.01955, p = 0.9807, n ≥ 9; Nep4A: F(2,32) = 1.609, p = 0.2168, n ≥ 9).