Chemicals reagents and drugs for primary neuronal culture
| Chemicals | Concentration | Supplier; catalog number |
|---|---|---|
| NaCl | 116 mm | Sigma-Aldrich; S7653 |
| KCl | 5.4 mm | Sigma-Aldrich; P9541 |
| NaHCO3 | 26 mm | Sigma-Aldrich; S5761 |
| d-(+)-glucose | 25 mm | Sigma-Aldrich; G8270 |
| NaH2PO4 | 2 mm | Sigma-Aldrich; 71505 |
| MgSO4 | 1 mm | Sigma-Aldrich; m7506 |
| Cysteine | 1.3 mm | Sigma-Aldrich; C6645 |
| EDTA | 0.5 mm | Sigma-Aldrich; E9884 |
| Kynurenate | 0.5 mm | Sigma-Aldrich; K3375 |
| Papain | 20 U/ml | Worthington; LS003126 |
| Penicillin/streptomycin | 7.70% | Sigma-Aldrich; P4333 |
| l-glutamine | 0.90% | Sigma-Aldrich; 59202C |
| Poly-i-lysine | 100 μg/ml | Sigma-Aldrich; P1399 |
| Laminin | 5 μg/ml | Sigma-Aldrich; L2020 |
| Insulin | 0.025 g/ml | Sigma-Aldrich; I2643 |
| Apotransferrin | 50 ml/ml | Sigma-Aldrich; T0178 |
| GDNF | 1 ng/ml | Sigma-Aldrich; SRP3200 |