Table 1.

Primers used for quantification of gene expression

GeneGene namePrimer directionPrimer sequenceAmplicon size (bp)Two-way ANOVA results (versus rps13 + rpl32)
TimeTreatmentInteraction
trαThyroid Hormone Receptor αForTCCAGACAGCGAGACCCTAA910.4490.8660.0053
RevGGCATCAGAGACAACACCCA
trβThyroid Hormone Receptor βForAGATCATGTCCCTCCGAGCA1140.0004<0.00010.0818
RevCCACACCGAGTCCTCCATTT
nrepNeuronal Regeneration Related ProteinForTGTAGCGGGAGCAATCACAA1620.0019<0.00010.0004
RevACATTCAGAAACCTGCCCCT
pcnaProliferating Cell Nuclear AntigenForTTCTTGTGCGAAGGATGGGG150<0.0001<0.00010.508
RevCGCAATGCAAATGTGAGCTG
mcm5Minichromosome Maintenance Complex Component 5ForGTGCTTGCTGCTTTCTTCTCT81<0.0001<0.00010.0207
RevCTCTCCGCCAAAACTATCGC
gapdhGlyceraldehyde-3-Phosphate DehydrogenaseForAATCTACTGGAGTCTTCACAACCA1390.6360.03090.135
RevGAGTTCTCATATTTCTCATGGTTCA
ywhazTyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, ZetaForAATGCCACGCAACCAGAAAG101<0.00010.00190.0276
RevATATCTGACTTTGCATCGCCG
hprt1Hypoxanthine Phosphoribosyltransferase 1ForATGCAGCCGATCTGGAGAAA720.1760.00090.77
RevCTCGAGCCAGCCTTTCAGTC
rps13Ribosomal Protein S13ForATGTCAAGGAACAGATCTTCAAACT131
RevGAGGATTCTCAGGATTTTATTACCA
rpl32Ribosomal Protein L32ForGCTCTTCATCGGGCTGTCTA77
RevCTTGGTGAGAGGTCTGAGGG
  • Forward and reverse primer sequences that were used for quantification of expression for specified genes are shown. Analysis using two-way ANOVA compared with the ribosomal proteins, rps13 and rpl132, is shown.