Table 2.

Candidates evaluated for use as reference genes

GenePercent difference of T3 from ControlTwo-way ANOVA resultsgeNorm M-valueNormFinder Stability value
Day 2Day 4TimeTreatmentInteraction
gapdh16.460.00.5760.0250.2660.6070.0186
ywhaz67.649.2<0.001<0.0010.1250.5680.0711
hprt154.9109.90.542<0.0010.4210.5630.0454
rps131.919.80.0120.5160.6270.7100.0819
rpl3213.930.00.0080.1110.430.7360.0692
  • Candidates were evaluated to determine if the genes respond to T3 treatment and for their stability as potential reference genes using geNorm and NormFinder analysis tools.